基于H2O2的NIR-II-响应性多功能纳米酶循环和破坏细胞逆氧稳态,用于增强协同作用的癌症治疗
Pinghua Ling1, Danjie Song1, Pei Yang1
1Laboratory of Functionalized Molecular Solids, Ministry of Education, Anhui Province Key Laboratory of Biomedical Materials and Chemical Measurement, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241002, China.
ACS biomaterials science & engineering
|July 16, 2024
概括
这项研究引入了一种新型纳米酶 (CSA),通过耗尽谷氨和产生活性氧物种 (ROS) 来破坏癌细胞的氧化还原平衡,为乳腺癌提供了一个有前途的协同疗法.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术 纳米技术
- 癌症治疗 癌症治疗
背景情况:
- 通过活性氧物种 (ROS) 扰乱细胞氧化还原稳定是癌症治疗的关键策略.
- 基于纳米酶的ROS生成具有前景,但受到催化活性和对过氧化或氧气的依赖所限制.
- 针对瘤微环境的抗氧化系统对于有效的癌症治疗至关重要.
研究的目的:
- 开发一种新型纳米酶 (CSA) 用于通过扰乱细胞氧化还原平衡来协同治疗乳腺癌.
- 为了增强ROS生成和治疗疗效超出目前的纳米酶限制.
- 研究氧化还原破坏,ROS生产和光热疗法的联合效应.
主要方法:
- 合成了一种纳米酶 (CSA),其中包括 CuSe 空心纳米立方体,涂有 Ag 纳米粒子.
- 研究了CSA与铁素减少酶 (TrxR) 和谷氨 (GSH) 的相互作用,以破坏抗氧化剂防御.
- 评估了CSA对ROS生成的过氧化酶模仿活性及其在NIR-II区域的光热性能.
主要成果:
- CSA有效地抑制了TrxR活性和耗尽了GSH,增加了细胞内ROS水平.
- CSA显示出高的过氧化酶模仿活性,产生基激素,超氧化激素和单片氧.
- 在CSA中的Ag纳米颗粒使得癌细胞中的高效光热转换和增强光催化ROS生成成为可能,从而导致显著的瘤生长抑制.
结论:
- 开发的CSA纳米酶有效地破坏细胞氧化还原稳定,并增强用于协同癌症治疗的ROS生成.
- 通过联合氧化还原调制,ROS催化和光热疗法,CSA表现出强大的抗癌作用.
- 这种纳米酶平台为克服当前基于ROS的癌症治疗方法的局限性提供了一个有希望的策略.
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