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物理细胞与细胞的接触会在葡萄酵母物种中引起特定的转录基因反应

Natasha A Luyt¹, Riaan N de Witt², Benoit Divol¹

¹Department of Viticulture and Oenology, South African Grape and Wine Research Institute, Stellenbosch University, Stellenbosch, Western Cape, South Africa.

Microbiology spectrum

|July 16, 2024

概括

酵母菌种Saccharomyces cerevisiae和Lachancea thermotolerans在对身体接触的反应中改变基因表达,影响细胞壁完整性和应激反应,为葡萄酒生态系统适应提供了洞察力.

科学领域:

微生物生态学微生物生态学
分子生物学分子生物学
酵母遗传学酵母遗传学

背景情况:

葡萄汁发酵涉及复杂的酵母和细菌生态系统.
酵母与酵母相互作用的分子机制,特别是身体接触,尚不清楚.
了解这些相互作用对于葡萄酒发酵和微生物生态学至关重要.

研究的目的:

为了研究Saccharomyces cerevisiae和Lachancea thermotolerans对物种间物理接触的转录基因反应.
阐明酵母对共同培养的反应背后的分子机制.
探索物理相互作用如何影响葡萄酒生态系统中的基因表达和适应.

主要方法:

在物理与代谢接触条件下对Saccharomyces cerevisiae和Lachancea热耐药的转录组分析.
定量PCR (qPCR) 用于验证基因表达变化.
对基因调节的比较分析,以应对特定物种的生物挑战.

主要成果:

身体接触诱导了S. cerevisiae细胞壁完整性和H2S生产基因的上调.
在L. thermotolerans中,HSP应激反应基因被显著上调.
这两种物种都降低了参与细胞粘附的FLO家族基因.
基因表达调节是特定于物种的,表明了适应性反应.

结论:

酵母物种积极调节基因表达,以应对物种间的物理接触.
这些转录变化对于适应葡萄酒生态系统和管理物种间竞争至关重要.
这些发现为酵母生态和进化适应提供了基本的见解,对生物技术应用有意义.

关键词:

在RNA-seqqq.细胞与细胞的相互作用.微生物生态学微生物生态学葡萄酒生态系统的生态系统酵母的进化适应酵母的进化适应酵母的转录基因反应

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