沙门氏菌Typhimurium对再暴露于次致命的西普罗素的动态反应
Jiseok Yi1, Junhwan Kim1, Juhee Ahn1,2
1Department of Biomedical Science, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea.
FEMS microbiology letters
|July 16, 2024
概括
暴露于低剂量西普罗夫洛克萨的沙门氏菌Typhimurium表现出改变的生长和运动性,表明历史依赖的行为. 这种抗生素预暴露诱导了细菌群体的交叉耐药性和附带敏感性.
科学领域:
- 微生物学 微生物学
- 细菌生理学 细菌生理学
- 抗生素耐药性 抗生素耐药性
背景情况:
- 低致死的抗生素度可以诱导复杂的细菌反应.
- 了解依赖于病史的细菌行为对于预测治疗结果至关重要.
研究的目的:
- 评估沙门氏菌 Typhimurium 在再暴露于次致命度的西普洛素时的历史依赖性行为.
- 评估西普洛素预暴露对细菌生长,恢复,健康和运动性的影响.
主要方法:
- 沙门氏菌Typhimurium菌株被预先暴露在不同度的西普洛素 (0, 1/16, 1/8, 1/4 MIC).
- 测量了细菌生长,抗生素后效应 (PAE),相对适应性,游泳运动性和滞后阶段持续时间 (LPD).
- 磁盘扩散测试确定了对多种抗生素的交叉耐药性和附带敏感性.
主要成果:
- 前期暴露于西普罗夫洛克萨导致增长延迟和细菌动态的改变.
- 在HIGH治疗组中观察到最高的PAE和细菌波动.
- 在HIGH治疗组中,观察到相对健身和游泳运动率的降低.
- 在Low治疗组中,滞后期持续时间在重新暴露后显著减少.
- 观察到对聚米辛B的交叉耐药性和对 ceftriaxone, erythromycin 和 gentamicin 的附带敏感性.
结论:
- 齐普罗夫洛克萨预暴露会诱导沙门氏菌Typhimurium的表型多样性和历史依赖的行为.
- 这些发现凸显了细菌群体在亚致命抗生素应激下具有动态性.
- 了解这些适应性反应对于管理抗生素耐药性至关重要.
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