脱离化蛋白涂层可以产生热稳定和内在无菌的生物配方
Harminder Singh1, Atip Lawanprasert1, Utkarsh1
1Department of Biomedical Engineering, Pennsylvania State University, University Park, Pennsylvania 16802-4400, United States.
ACS applied materials & interfaces
|July 16, 2024
概括
研究人员开发了新的化工添加剂,以稳定非水性溶剂中的蛋白质,使其能够在高温下储存,并防止微生物污染,用于强大的蛋白质试剂和疗法.
科学领域:
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
背景情况:
- 热无活化限制了蛋白质试剂和治疗稳定性,影响了生物活性并增加了污染风险.
- 目前的储存方法依赖于水溶液,水溶液易受温度波动和微生物生长的影响.
研究的目的:
- 在溶剂中设计用于稳定蛋白质分散的化学添加剂.
- 为了使蛋白质能够在极端温度 (≥90°C) 中保存,并防止微生物污染.
主要方法:
- 能与蛋白质表面结合的化学添加剂的合理设计.
- 蛋白质分散在化介质中而不是水溶液中.
- 在动物模型中评估蛋白质稳定性,生物活性和生物可用性.
主要成果:
- 用化物涂覆的蛋白质在溶剂中的温度≥90°C时保持稳定和功能.
- 性蛋白质配方抵抗了细菌,真菌和病毒的污染.
- 功能化的蛋白质显示出与盐水配方相当的血清生物可用性,并且没有改变*in vivo*的药理特征.
结论:
- 使用化工添加剂的非水性蛋白质存储范式增强了热稳定性并防止了污染.
- 这一战略为开发保质性蛋白试剂和生物制药提供了一个有前途的方法.
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