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什么样的精子参数会影响胚胎细胞的形成和质量在ICSI期间,严重的男性不孕不育

Weihua Jiang^1,2, Lihua Zhu^1,2, Lina Gao^1,2

¹Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China.

Systems biology in reproductive medicine

|July 16, 2024

概括

在严重的男性不孕症病例中,经过细胞体内精子注射 (ICSI),精子DNA碎片化 (DFI) 和形态学会影响胚胎质量. 精子质量的降低与胚胎细胞的发育和质量的降低有关.

科学领域:

生殖生物学生殖生物学
人类胚胎学人类胚胎学
不孕症的研究研究.

背景情况:

精子DNA完整性和形态对于胚胎发育至关重要.
严重的男性不孕症在像ICSI这样的辅助生殖技术中提出了独特的挑战.
了解影响囊胚形成的精子参数对于优化ICSI结果至关重要.

研究的目的:

调查特定精子参数对ICSI周期中囊胞形成率的影响,包括严重的男性不孕症.
分析精子DNA碎片化 (DFI) 和精子形态与胚胎细胞发育和质量之间的相关性.

主要方法:

追溯分析296个ICSI周期的严重的oligoasthenoteratozoospermia (OAT) 和99个周期的preimplantation基因测试,以检测阳 (PGT-A).
在OAT ICSI子组内进行相关性分析和数据分层.
评估胚芽细胞形成率,第5天和第6天的高质量胚芽细胞率,以及包括DFI和形态在内的精子参数.

主要成果:

在OAT ICSI周期中,胚芽细胞的形成和质量与精子参数差异有关.
精子DNA碎片化 (DFI) 和形态学显著影响了胚胎细胞形成率和第6天的高质量胚胎细胞率,但不是第5天的发育.
在正常DFI的类精子患者中,在第6天观察到更高质量的芽细胞囊,而正常形态学在DFI子组中没有显著差异.

结论:

降低精子质量,特别是降低DFI和改善形态,在ICSI周期中对囊胚质量和发育产生积极影响.
精子DNA碎片化和形态是预测严重男性不孕症中的胚胎细胞质量的关键指标.
根据DFI和形态学优化精子选择可能会提高ICSI成功率.

关键词:

DNA碎片化指数 (DFI) 是一个指数.美国国务院调查局 (ICSI)精子形态精子形态高品质的胚胎囊细胞比率.胚胎囊的形成速度.

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