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在形态遗传组织流动之前和期间,E-cadherin调整组织机械行为

Xun Wang¹, Christian M Cupo¹, Sassan Ostvar¹

¹Department of Mechanical Engineering, Columbia University, New York, NY 10027, USA.

Current biology : CB

|July 16, 2024

概括

由E-cadherin调节的上皮细胞粘附,在发育过程中影响组织力学和细胞流动. 改变E-cadherin影响细胞形状和重新排列速度,揭示了组织动态中的双重作用.

科学领域:

发展生物学发展生物学
细胞生物学细胞生物学
生物物理学的生物物理.

背景情况:

表皮组织表现出复杂的机械行为,对发育至关重要.
细胞-细胞粘附,特别是通过E-cadherin,对于上皮组织完整性和机制至关重要.
在像形态生成这样的动态过程中,粘附对组织机制的确切影响仍然不完全理解.

研究的目的:

研究E-cadherin介导的粘附如何影响表皮组织在形态发生过程中的机械行为.
分析E-cadherin在静态和动态流动的交汇表皮组织中的作用.
在体内阐明细胞粘附,组织结构和细胞流动之间的关系.

主要方法:

系统调节Drosophila胚胎的生殖带表皮中的E-cadherin水平.
在体轴延长之前和期间分析组织力学,细胞形状和细胞流动.
探究依赖于actomyosin的力量及其与E-cadherin水平的关系.

主要成果:

在轴延长之前,增加的E-cadherin水平导致了更长的细胞和更类似流体的组织状态,减少了对流动的阻力.
在轴延长过程中,E-cadherin主要调整了细胞重组事件的速度.
乙素水平影响了依赖于actomyosin的力量,这表明它在调整组织力学方面发挥了作用.
在E-cadherin的显著变化 (约. 4倍) 对整体组织结构和流动的影响相对较弱,表明系统的稳定性.

结论:

在控制上皮质组织结构和动态方面,E-cadherin起着双重,有时是相反的作用.
粘附通过调节细胞形状,流动和actomyosin活动来影响组织力学.
流性上皮组织表现出对功能范围内的E-cadherin水平变化的耐受性.
这些发现揭示了细胞粘附和组织流动之间的复杂,非直观的关系 in vivo.

关键词:

这是E-cadherin.细胞对细胞的粘附.收延伸的收延伸是什么意思皮质形态发生的表皮形态发生.组织流动性组织流动性

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