在酵母中引入碳同化,使用光合作用指导的内生共生
Yang-le Gao1, Jay Cournoyer1, Bidhan C De1
1Department of Chemistry, University of Illinois at Urbana-Champaign, 600 S Mathews Avenue, Urbana, Illinois, US.
Nature communications
|July 16, 2024
概括
研究人员通过整合蓝色细菌创造了光合作用酵母,使二氧化碳同化和自然产品生物合成能够在没有传统糖的情况下进行. 这一突破推动了可持续生物技术和合成生物学.
科学领域:
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
- 生物技术是生物技术.
背景情况:
- 将异质性生物转化为自营性生物通常需要广泛的代谢工程.
- 目前的方法主要集中在将二氧化碳 (CO2) 导入中央碳代谢中.
研究的目的:
- 开发一种新的指导性内共生方法,以引入碳同化到芽的酵母.
- 为了设计能够进行光合作用和生物合成的酵母/蓝色细菌嵌合体.
主要方法:
- 工程化碳同化和糖分泌的光合成蓝藻细菌作为酵母细胞内的内共生生物.
- 在光合作用条件下使用二氧化碳作为碳源的培养酵母/菌仿真体.
- 在工程化梅拉中证明了天然产品的生物合成.
主要成果:
- 成功生成了稳定的酵母/菌奇默体,可以在没有外部碳来源的情况下通过光合作用传播.
- 展示了这些嵌合体在自的条件下生物合成自然产品的能力.
- 将内共生方法扩展到标准的实验室酵母菌株,创造了光合作用木乃伊.
结论:
- 定向内共生提供了一个强大的策略,用于创建自营酵母.
- 这种方法对可持续生物技术和合成生物学有重大影响.
- 工程化梅拉可以作为研究新有机体进化适应的模型.
相关概念视频
The Calvin Benson Cycle
4.5K
Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...
4.5K
What is Photosynthesis?
98.9K
Photosynthesis is a multipart, biochemical process that occurs in plants as well as in some bacteria. It captures carbon dioxide and solar energy to produce glucose. Glucose stores chemical energy in the form of carbohydrates. The overall biochemical formula of photosynthesis is 6 CO2 + 6 H2O + Light energy → C6H12O6 + 6 O2. Photosynthesis releases oxygen into the atmosphere and is largely responsible for maintaining the Earth’s atmospheric oxygen content.
98.9K
Yeast Signaling
14.6K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
14.6K
The Calvin Cycle
73.8K
Overview
73.8K
C4 Pathway and CAM
45.4K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
C4 Pathway
The C4 pathway is used by plants such as...
45.4K
The Anatomy of Chloroplasts
5.1K
Green algae and plants, including green stems and unripe fruit, harbor specialized organelles called chloroplasts to carry out photosynthesis. They coordinate both stages of photosynthesis — the light-dependent reactions and the light-independent reactions. The light-dependent reactions use sunlight to release oxygen and produce chemical energy in the form of ATP and NADPH, and the light-independent reactions capture CO2 and use ATP and NADPH to produce sugar.
Structure of...
Structure of...
5.1K


