使用三酸提取和多孔石墨碳静止相的极地代谢学
Francesca Day1, Justin O'Sullivan1,2,3,4,5, Farha Ramzan1
1Liggins Institute, The University of Auckland, Auckland, New Zealand.
概括
这项研究引入了一种新的液态染色体质谱法 (LC-MS) 方法,用于准确识别和量化非目标代谢学中的极性代谢物. 该方法提供可靠的保留和有效的恢复,改善极地代谢组的覆盖范围.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 代谢学 代谢学 代谢学
背景情况:
- 与非极地代谢物相比,非向代谢学在准确识别和量化极地代谢物方面面临着挑战.
- 目前的方法通常依赖于水友相互作用色谱学和气色谱学-质谱学进行极性代谢物分析.
研究的目的:
- 展示一种简单的,单步提取方法与液态染色学质谱学 (LC-MS) 结合,可靠地保持极性代谢物.
- 建立一个有效和准确的方法来量化在更广泛的代谢组内的极性代谢物.
主要方法:
- 从人体血清中使用MilliQ + 10%三酸的一步提取.
- 使用有孔的石墨碳液态染色体质谱法 (LC-MS).
- 用量化,保留和系统适用性 (QreSS) 的内部标准来评估方法的一致性和恢复.
主要成果:
- 极性代谢物的可靠保留 (CV <0.30) 在一个广泛的logP范围 (-9.1至5.6).
- 通过QreSS标准确认的极地到中极地代谢物的一致性 (CV<0.16) 和有效恢复 (70-130%) 的表现.
- ~80%的注释代谢物在质量控制系列中得到准确量化 (皮尔森r > 0.80).
结论:
- 与现有技术相比,开发的LC-MS方法提供了极性代谢组的优越覆盖.
- 这种方法提供了一种可靠和有效的手段,用于在非向的代谢学研究中准确量化极性代谢物.
更多相关视频
09:38Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
Published on: January 7, 2019
8.6K
09:01Author Spotlight: Separation of Coral Host Tissues and Algal Symbionts and Analyzing Their Metabolites
Published on: October 13, 2023
1.9K
相关概念视频
01:17Gas Chromatography: Types of Columns and Stationary Phases
563
Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
563
01:09Analyte Adsorption and Distribution
631
In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and...
631