通过在雄和雌之间进行多组织转录组比较,识别肌肉发育中的候选基因和生物途径
Yunzhou Yang1, Cui Wang1, Shufang Chen2
1Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Science, Shanghai, 201106, China.
Scientific reports
|July 16, 2024
概括
性行为显著影响东白的生长. 转录组分析揭示了肌肉和垂体组织中的关键基因和途径,为性别驱动的发育差异提供了洞察力.
科学领域:
- 基因组学就是基因组学.
- 动物科学动物科学
- 发展生物学 发展生物学
背景情况:
- 体重和身高的两性变异很常见,但潜在的遗传机制尚未完全理解.
- 东白 (ZDW) 由于选择性繁殖而表现出两性之间显著的体重差异,这使得它们成为研究受性别影响成长的有价值模型.
研究的目的:
- 研究驱动ZDW的性别特异生长差异的分子机制.
- 通过比较雄性和雌性之间的肌肉和垂体组织的转录组来确定参与生长和发育的关键基因和途径.
主要方法:
- 腿部肌肉,乳腺肌肉和来自雄性和雌性ZDW的垂体腺组织的比较转录组分析.
- 基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径丰富分析.
- 蛋白质与蛋白质相互作用网络分析.
主要成果:
- 在所有三种组织中确定了大量的差异表达基因 (DEGs),在两性之间具有明显的上调和下调模式.
- 丰富分析显示,DEGs参与内分泌代谢,肌肉形成和骨发育途径.
- 男性偏向的基因表达与营养消化,吸收和消化汁分泌途径有关.
结论:
- 转录组分析提供了对性别驱动对生长和发育的影响的全面了解.
- 像IGF1,GHRHR和NCAPG-LCORL这样的基因,以及与肌肉发育相关的途径,可能对生长中的性别差异至关重要,可能在荷尔蒙影响之前起作用.
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