不同碳和来源对Aureobasidium pullulansNBRC 100716菌株氨酸生产动力学的影响
Aslı Deniz Pehlivan1, M Tijen Bozdemir2, Z Yesim Ozbas3
1Faculty of Engineering, Department of Food Engineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey.
Archives of microbiology
|July 16, 2024
概括
这项研究利用Aureobasidium pullulans NBRC 100716优化了氨酸的生产,将果糖和酸酵母提取物确定为理想的营养素. 生产的氨酸表现出强大的生物表面活性剂特性和独特的脂肪酸特征.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 生物化学 生物化学
背景情况:
- 氨酸是来自Aureobasidium pullulans的多脂,具有宝贵的抗菌,抗癌和表面活性物质.
- 潜在的应用范围涵盖食品,农业,医疗和制药行业,推动人们对优化其生产的兴趣.
研究的目的:
- 调查各种碳和源对A. pullulans生长和氨酸生产动态的影响NBRC 100716.
- 描述在最佳条件下生产的氨酸的生物表面活性剂能力和脂肪酸成分.
主要方法:
- 对六种A. pullulans菌株进行选,以生产氨酸.
- 用不同的碳和来源在水浴中进行发酵实验.
- 分析了氨酸产量,特异性生产率,表面张力降低和脂肪酸概况.
主要成果:
- 作为碳源的果糖和作为源的顿酵母提取物混合物显著增强了氨酸的生产.
- A. pullulans NBRC 100716 取得了4.26g/L的最大产量,其特异性生产率为0.0090g/g/h.
- 生产的氨酸显示出出色的生物表面活性剂活性,降低了与合成表面活性剂相比的水面张力.
- 氨酸的脂肪酸概况显示了大量的不和脂肪酸含量,类似于植物油.
结论:
- 使用果糖和酸酵母提取物的优化发酵条件使得A. pullulans NBRC 100716能够生产高产的氨酸.
- 这种菌株产生的氨酸具有强大的生物表面活性剂特性和有利的脂肪酸成分,可用于潜在的工业应用.
- 这项研究建立了使用果性A. pullulans菌株生产氨酸的新方法,为其商业化开辟了道路.
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