相关实验视频

Updated: Jun 20, 2025

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

Published on: May 3, 2021

一个新的预后签名与骨髓瘤中编程细胞死亡有关

Yu-Chen Jiang¹, Qi-Tong Xu², Hong-Bin Wang¹

¹Affiliated Zhongshan Hospital Of Dalian University, Dalian, China.

Frontiers in immunology

|July 17, 2024

概括

这项研究确定了关键的编程细胞死亡 (PCD) 基因,以创建骨髓瘤编程细胞死亡评分 (OS-PCDS),以改善骨髓瘤预后. 该OS-PCDS模型准确地预测了患者的结果,并突出了潜在的治疗目标.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

骨髓瘤 (OS) 是患有预后不佳的儿童和青少年常见的骨癌.
编程细胞死亡 (PCD) 越来越多地被认为在瘤发育和进展中的作用.
目前对OS的预后评估需要加强,以改善患者的治疗结果.

研究的目的:

在骨髓瘤中识别与细胞死亡相关的新型预后风险基因.
使用机器学习构建和验证骨髓瘤强大的预后风险模型.
阐明已识别的基因在骨髓瘤细胞行为中的功能性作用.

主要方法:

从TARGET和GEO数据库收集和分析骨髓瘤患者的数据.
从文献和数据库中整理了1621个编程细胞死亡 (PCD) 基因.
整合了十个机器学习算法来构建骨髓瘤编程细胞死亡得分 (OS-PCDS) 模型.
使用独立数据集验证了模型,并进行了基因淘汰实验.

主要成果:

确定了30个与骨髓瘤中PCD相关的预后风险基因.
使用随机生存森林 (RSF) 算法开发了一个最佳的OS-PCDS模型,包括五个关键基因:MTM1,MLH1,CLTCL1,EDIL3和SQLE.
在骨髓瘤细胞中观察到这些基因的mRNA和蛋白质表达的显著变化.
证明了CLTCL1,EDIL3和SQLE的淘汰会影响骨髓瘤细胞的增殖,迁移和亡.

结论:

使用五个特定基因的OS-PCDS模型,准确地预测骨髓瘤的预后.
这些已识别的基因 (CLTCL1,MTM1,MLH1,EDIL3,SQLE) 在骨髓瘤病理生理学中起着至关重要的作用.
这些基因代表了潜在的治疗标和骨髓瘤的预后生物标志物.

关键词:

机器学习算法机器学习算法骨质肉瘤是骨质肉瘤的一种疾病.预后标志物是一个预后标志物.被编程的细胞死亡.瘤免疫学瘤免疫学

更多相关视频

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts

Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts

Published on: July 25, 2020

相关概念视频

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...