Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Flow Cytometry01:23

Flow Cytometry

12.6K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
12.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Changes in Upper Airway Anatomy and Apnea-Hypopnea Index in Patients with Obstructive Sleep Apnea Undergoing Bariatric Surgery: A Pilot Study.

Journal of clinical medicine·2026
Same author

Splenic Abscess in Patients With Prior History of Enteric Fever: A Case Series.

Cureus·2026
Same author

Treatment Algorithm for Patients with Obesity in India: A Joint Consensus by Endocrine Society of India and Obesity Surgeons Society of India.

Obesity surgery·2026
Same author

Long-term nutritional outcomes after bariatric surgery: A multicentre study by the Obesity and Metabolic Surgery Society of India.

Journal of minimal access surgery·2026
Same author

Pet dogs supporting caregivers of children with special needs.

Bioinformation·2026
Same author

Molecular and Pharmacogenetic Marker Evaluation in Relation to the Toxicity and Clinical Response of Acute Lymphoblastic Leukemia Treatment in Indian Children (MPGx-INDALL): Protocol for a Prospective Observational Cohort Study.

JMIR research protocols·2026
Same journal

Investigating the interactomic landscape of survival motor neuron (SMN) and the SMNΔ7 truncated protein.

BioTechniques·2026
Same journal

Antigen retrieval-immunofluorescence on free floating sections to visualize the liver lobule and its cellular makeup.

BioTechniques·2026
Same journal

Special approach of droplet digital polymerase chain reaction (ddPCR) for transgene stability of a Chinese hamster ovary (CHO) cell line.

BioTechniques·2026
Same journal

Strand-specific quantification of L1 ORF0 and related transcripts by multiplex reverse transcription with tagged primers.

BioTechniques·2026
Same journal

Why and when should we choose digital PCR?

BioTechniques·2026
Same journal

Quantitative and unbiased lung alveolar septum assessment in an LPS experimental mouse model using 2D-spatial correlation image analysis from hematoxylin and eosin slides.

BioTechniques·2026
查看所有相关文章

相关实验视频

Updated: Jun 20, 2025

Isolation of Adipose Tissue Immune Cells
07:09

Isolation of Adipose Tissue Immune Cells

Published on: May 22, 2013

36.2K

使用流细胞计进行脂肪细胞ABCA1表达分析.

Sakshi Shukla1, Ashutosh Bansal2, Sandeep Aggarwal3

  • 1Department of Biochemistry, AIIMS, New Delhi, India.

BioTechniques
|July 17, 2024
PubMed
概括
此摘要是机器生成的。

这项研究提出了一种改进的流细胞测量协议,用于表征脆弱的脂肪细胞,即使来自高BMI受试者. 优化的方法提高了细胞活力和膜蛋白分析,提高了可行性和成本效益.

关键词:
在ABCA1中,ABCA1是ABCA1.这就是为什么BMI是BMI.脂肪细胞 (adipocytes) 是一种脂肪细胞.脂肪组织的脂肪组织.流动细胞计量是流动细胞计量的方法.门 门 门 门 这是什么意思脂质滴滴滴滴滴滴滴滴滴滴滴滴滴

更多相关视频

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry
07:32

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

Published on: March 6, 2018

17.5K
Preparation of Adipose Progenitor Cells from Mouse Epididymal Adipose Tissues
06:17

Preparation of Adipose Progenitor Cells from Mouse Epididymal Adipose Tissues

Published on: August 25, 2020

5.7K

相关实验视频

Last Updated: Jun 20, 2025

Isolation of Adipose Tissue Immune Cells
07:09

Isolation of Adipose Tissue Immune Cells

Published on: May 22, 2013

36.2K
Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry
07:32

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

Published on: March 6, 2018

17.5K
Preparation of Adipose Progenitor Cells from Mouse Epididymal Adipose Tissues
06:17

Preparation of Adipose Progenitor Cells from Mouse Epididymal Adipose Tissues

Published on: August 25, 2020

5.7K

科学领域:

  • 细胞生物学 细胞生物学
  • 生物技术是生物技术.
  • 生物化学 生物化学

背景情况:

  • 通过流细胞测量来表征脂肪细胞是困难的,因为细胞脆弱性,特别是在高体重指数 (BMI) 的个体.
  • 现有的方法往往导致细胞破裂和不准确的膜蛋白分析.

研究的目的:

  • 为可靠的脂肪细胞膜蛋白质分析开发一个优化的流动细胞计量协议.
  • 为了克服与脆弱脂肪细胞和高BMI样本相关的挑战.

主要方法:

  • 优化了组织消化时间和减少了中间步骤,以尽量减少脂肪细胞摩擦和破裂.
  • 实施了修改后的封闭策略,以排除脂质滴,确保精确的细胞群体分析.
  • 通过检查膜蛋白ABCA1.1的表达水平来验证脂肪细胞选择.

主要成果:

  • 该协议成功地在门区内维持了高达90%的可活细胞群.
  • 证明有效的脂肪细胞选择和膜蛋白评估.
  • 该方法需要较小的组织样本,提高可行性和成本效益.

结论:

  • 这种优化的流式细胞计量协议为研究脂肪细胞膜特征提供了强大的和高效的方法.
  • 该方法提高了脂肪细胞的细胞活力和分析精度,特别是在具有挑战性的样本类型中.
  • 这一进步有助于更好地研究脂肪细胞功能和相关的代谢条件.