丸细胞中的长非编码RNA (LINC-RBE) 表达与老鼠的衰老有关
Ajay Kumar Danga1, Sukhleen Kour1,2, Anita Kumari1
1Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.
Biogerontology
|July 17, 2024
概括
长非编码RNAs (lncRNAs) 调节细胞过程. 这就是LINC-RBERNA.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 老龄化导致生理衰退和与年龄有关的疾病.
- 哺乳动物的丸产生雌性体和雌激素,这对繁殖至关重要.
- 长非编码RNAs (lncRNAs) 是细胞功能的关键调节者.
研究的目的:
- 研究LINC-RBERNA在老化过程中在老鼠丸中的作用.
- 确定LINC-RBE在不同丸细胞类型和年龄的表达模式.
主要方法:
- 在不同年龄 (4,16,44和70周) 的老鼠丸中对LINC-RBERNA表达的定量分析.
- 评估丸细胞内的LINC-RBERNA局部化.
主要成果:
- 成年大鼠的LINC-RBERNA表达达到峰值,未成熟和老年大鼠的表达下降.
- 在莱迪格细胞,初级精子细胞和塞尔托利细胞中观察到最高的LINC-RBE表达.
- 观察到LINC-RBE表达和局部化的与年龄相关的变化.
结论:
- 在老鼠丸中,LINC-RBE RNA的表达是发育调节和年龄依赖的.
- 衰退的LINC-RBE表达与老年大鼠的丸生殖功能下降相关.
- LINC-RBE在丸生理学中起着重要作用,特别是在莱迪格细胞,初级精子细胞和塞尔托利细胞中.
相关概念视频
02:39lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
02:39lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
03:18Non-LTR Retrotransposons
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...