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相关概念视频

Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

562
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
562

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相关实验视频

Updated: Jun 20, 2025

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
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人类B细胞受体谱的序列测序.

Prasanti Kotagiri1, Rachael J M Bashford-Rogers2,3, Vanessa L Bryant4,5,6

  • 1Immunology Alfred Hospital, Central Clinical School, Monash University, Melbourne, VIC, Australia. prasanti.kotagiri@monash.edu.

Methods in molecular biology (Clifton, N.J.)
|July 17, 2024
PubMed
概括
此摘要是机器生成的。

高通量测序揭示了B细胞受体 (BCR) 的复杂性,但面临偏差. 这项研究优化了BCR图书馆的准备,以便在健康和疾病中准确,可重现的分析B细胞免疫力.

关键词:
在B细胞内存中,B细胞的记忆B细胞受体受体 B细胞受体受体在BCR的曲目中,BCR的曲目目目录.细胞分类 细胞分类人类B细胞是人类B细胞.下一代测序测序是什么

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T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
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VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma
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VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma

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相关实验视频

Last Updated: Jun 20, 2025

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
08:51

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

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VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma
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VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma

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科学领域:

  • 免疫学 免疫学 免疫学
  • 基因组学就是基因组学.
  • 分子生物学分子生物学

背景情况:

  • 下一代测序 (NGS) 提供了对B细胞受体 (BCR) 谱的洞察.
  • 目前用于BCR目录分析的NGS方法存在定量偏差和排序错误.
  • 这些局限性损害了免疫分析的准确性.

研究的目的:

  • 开发一个优化的协议,为人类的BCR目录图书馆准备.
  • 为了最大限度地减少偏差并提高BCR曲目高通量测序的准确性.
  • 为了使B细胞免疫的可复制分析.

主要方法:

  • 开发一种用于人类BCR的新型图书馆准备协议.
  • 实施质量控制步骤以减轻偏差.
  • 使用高通量测序对协议的验证.

主要成果:

  • 优化的协议显著减少了BCR目录数据的定量偏差.
  • 测序错误率被最小化,提高了数据可靠性.
  • 生成可复制和准确的人类BCR曲目档案.

结论:

  • 优化的协议为研究B细胞免疫提供了一个强大的方法.
  • 准确的BCR曲目分析对于了解健康和疾病状态至关重要.
  • 这种方法有助于免疫学研究和诊断的进步.