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相关概念视频

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

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Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
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相关实验视频

Updated: Jun 20, 2025

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
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通过一步的ATAC-seqq测量B细胞染色质可访问性.

Sakeenah L Hicks1, Christopher D Scharer2

  • 1Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, USA.

Methods in molecular biology (Clifton, N.J.)
|July 17, 2024
PubMed
概括

检测转移酶可访问色素 (ATAC) -seq协议有效地映射可访问的色素. 这种方法使用Tn5转基因酶进行DNA碎片化和适配器结合,从最小的细胞输入创建序列库.

关键词:
ATAC-seqq 的使用情况.染色体的可访问性 染色体的可访问性在表观遗传学上,表观遗传学是表观遗传学.基因调节 基因调节测序图书馆的序列化在Tn5转移酶中.

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相关实验视频

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科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 了解染色体可访问性对于研究基因调节至关重要.
  • 现有的方法通常需要大量的细胞数量.
  • 需要有效的染色体映射技术.

研究的目的:

  • 为了呈现一个优化的测试转移酶可访问性染色体 (ATAC) -seq协议.
  • 通过有限的细胞输入来实现可访问的染色质的全球映射.

主要方法:

  • 使用Tn5转基因酶同时进行DNA碎片化和适配器标记.
  • 在PCR放大过程中使用双索引开头.
  • 从原子核转换生成尺寸特定的测序库.

主要成果:

  • 该协议优化用于生成可访问色素的全球地图.
  • 它有效地使用一系列有限的细胞输入.
  • 概述了一个简化的一步工作流程.

结论:

  • 优化的ATAC-seq协议为染色质可访问性研究提供了强大的工具.
  • 它允许使用最小的生物材料进行高分辨率的基因组分析.
  • 这种方法促进了全面的表观遗传分析.