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通过PCR和桑格测序识别IgG1和IgG3全型

L Carissa Aurelia1, Ruth A Purcell1, Amy W Chung2

  • 1Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC, Australia.

Methods in molecular biology (Clifton, N.J.)
|July 17, 2024
PubMed
概括

这项研究详细介绍了通过分析免疫球蛋白重常量马 (IGHG) 基因群中的单核酸多态 (SNP) 来识别免疫球蛋白G (IgG) 类型的协议. 该方法使用聚合酶链反应 (PCR) 和桑格测序来准确确定全型.

关键词:
所有型类型 类型类型一个巨大的IGHG.在IgG IgG的基础上.在IgG1的基础上,IgG1在IgG3中,IgG3是IgG3.免疫遗传学 免疫遗传学这是一个PCRPCR.桑格尔测序是什么意思

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Sanger Sequencing01:57

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...