基于固体相提取和衍生产的GC-MS方法的开发和验证,用于分析生物样本中的自由和葡萄糖合双F
Xu-Liang Cao1, Svetlana Popovic1, Santokh Gill2
1Food Research Division, Bureau of Chemical Safety, Health Canada, Ottawa, ON K1A 0K9, Canada.
Journal of AOAC International
|July 17, 2024
概括
一种新的GC-MS方法准确地测量肝脏组织中的双F (BPF),检测自由形式和结合形式. 这种分析对于了解BPF的毒性至关重要,因为暴露水平的增加.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
- 毒理学 毒理学 毒理学
背景情况:
- 双F (BPF) 是BPA的潜在替代品,毒性数据有限.
- 在结构上,BPF与BPA相似,并且可能表现出类似的雌激素活性.
- 由于其潜在的广泛使用,了解BPF的毒性至关重要.
研究的目的:
- 开发和验证一种GC-MS方法,用于分析肝脏样本中的BPF.
- 量化自由和与葡萄糖合的BPF.
- 支持正在进行的BPF毒理学研究.
主要方法:
- 固体相提取 (SPE) 和导化用于样品制备.
- 气体染色学-质谱学 (GC-MS) 用于检测BPF.
- 使用β-glucuronidase进行酶去结合,以测量总BPF.
主要成果:
- 经过验证的方法表明,自由BPF (90-97.5%) 和葡萄糖合BPF (72.3-93.3%) 的恢复率很高.
- 在所有剂量大鼠肝样本中检测到BPF,在较高剂量时水平增加.
- 结合BPF的比例差异很大 (9.8%77.9%),突出显示需要测量两种形式.
结论:
- 成功开发并验证了一种强大的GC-MS方法,用于在肝脏样本中分析自由和葡萄糖合的BPF.
- 这项研究提供了分析单-β-D-葡萄胺结合的BPF的首次验证,证实了酶解结合的效率.
- 该方法可适应在其他生物矩阵中分析BPF,并经过适当的验证.
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