在近排卵期的人类和老鼠积卵细胞复合体中的蛋白酶表达:在积卵细胞复合体迁移中的作用†
Ketan Shrestha1, Muraly Puttabyatappa1, Michelle A Wynn1
1Department of Obstetrics and Gynecology, Chandler Medical Center, University of Kentucky, Lexington, KY, USA.
Biology of reproduction
|July 17, 2024
概括
这项研究表明,蛋白酶及其抑制剂对于排卵期间的积卵细胞复合体 (COC) 迁移能力至关重要. 这项研究强调了这些分子在使COC迁移成为可能的作用,这是排卵过程中的一个关键事件.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 积卵细胞复合体 (COC) 的迁移和矩阵入侵能力对于排卵至关重要.
- 虽然蛋白酶与癌细胞入侵有关,但它们在COC中的作用不太清楚.
- 关于在排卵过程中COC中蛋白酶表达的信息有限.
研究的目的:
- 研究大鼠和人类的COC中的蛋白酶和蛋白酶抑制剂的表达.
- 确定这些分子在排卵期间COCs迁徙表型中的作用.
- 为了比较COC和颗粒细胞之间的蛋白酶表达.
主要方法:
- 研究了老鼠和人类COC中的特定蛋白酶 (等离子体,MMP,ADAMTS) 和抑制剂 (TIMPs,SERPINE1) 的表达.
- 使用体内 (hCG 给药) 和体外 (弗斯科林,安菲瑞古林) 方法来诱导大鼠的COCs中的排卵类变化.
- 从排卵或试管婴儿检索中接受排卵或试管婴儿检索的老鼠和人类的COC和粒状细胞之间的基因表达比较.
- 在试验室中评估了矩阵金属蛋白酶抑制剂 (GM6001) 对COC细胞迁移的影响.
主要成果:
- 在老鼠中使用hCG增加了MMP,ADAMTS1和蛋白酶抑制剂 (TIMP1,TIMP3,SERPINE1) 的COC表达.
- 试管体内治疗模仿了这些变化,诱导MMPs,ADAMTS1和大鼠COCs中的抑制剂.
- 与颗粒细胞相比,人类的COC显示MMP1,MMP2,MMP9和ADAMTS1减少,但MMP16,TIMP1和TIMP3增加.
- 抑制矩阵金属蛋白酶在体外显著降低了累积细胞迁移.
结论:
- 多个蛋白酶及其抑制剂在卵巢周期间以COC表达.
- 这些蛋白酶和抑制剂可能参与了COCs的迁徙表型的传递.
- 这些发现提供了证明蛋白酶活性在排卵期间COC迁移中的功能重要性的证据.
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