使用CRISPR/Cas9与RNASeq结合的PLA2删除突变揭示了与eicosanoid信号传递相关的昆虫免疫基因
Mohammad Vatanparast1,2, Mojtaba Esmaeily1, David Stanley3
1Department of Plant Medicals, Andong National University, Andong, Korea.
PloS one
|July 17, 2024
概括
对于昆虫免疫力至关重要的类素,由脂酶A2 (PLA2) 合成. 破坏Sep-sPLA2基因在Spodoptera exigua显著损害了免疫反应和发育.
科学领域:
- 昆虫免疫学 昆虫免疫学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 伊可索诺伊德在调解昆虫的免疫反应中起着至关重要的作用.
- 分泌脂酶A2 (sPLA2) 独特地参与了类昆虫Spodoptera exigua的免疫力.
研究的目的:
- 为了调查Spodoptera exigua分泌脂酶A2 (Se-sPLA2) 在免疫反应中的作用.
- 了解eicosanoids对基因表达和昆虫整体健康的影响.
主要方法:
- 使用CRISPR/Cas9基因组编辑,在Spodoptera exigua中产生了一个Se-sPLA2删除突变.
- 在Illumina-HiSeq平台上的RNA测序 (RNASeq) 用于在免疫挑战后比较野生和突变系的转录组.
- 对12878个单基因进行了差异基因表达分析.
主要成果:
- 在S. exigua幼虫中,超过69%的表达基因在Se-sPLA2基因中断后被eicosanoids调节.
- 在S. exigua中,大约36%的免疫相关基因由eicosanoids控制.
- 这种Se-sPLA2缺失突变体在细胞和幽默反应中表现出显著的免疫抑制,以及减少的发育和生殖潜力.
结论:
- 通过Se-sPLA2合成的Eicosanoids是Spodoptera exigua免疫力的关键调节者.
- 破坏Se-sPLA2会导致广泛的免疫抑制,并对昆虫的发育和繁殖产生负面影响.
更多相关视频
07:46CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
Published on: December 11, 2020
5.8K
06:37Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner
Published on: July 1, 2021
4.4K
相关概念视频
01:59CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
02:53CRISPR and crRNAs
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
01:28CRISPR/Cas9 Genome Editing
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...