miR-129-2-3p与SEMA4C结合,以调节HCC的发展,并抑制EMT
Mutation research
|July 17, 2024
概括
微RNA-129-2-3p通过向SEMA4C.抑制肝细胞癌 (HCC). 这一发现揭示了HCC治疗的新治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 肝细胞癌 (HCC) 是最常见的初级肝癌.
- 微RNAs (miRNAs) 是小型非编码RNAs,调节基因表达,并参与各种生物过程,包括癌症.
研究的目的:
- 研究miR-129-2-3p在HCC中的作用.
- 探索在HCC中miR-129-2-3p和SEMA4C之间的关系.
主要方法:
- 定量实时PCR (RT-qPCR) 用于miRNA和mRNA的表达.
- 细胞增殖,入侵和迁移试验 (CCK-8,伤口愈合,转井).
- 西部涂抹 (WB),ELISA,RNA免疫沉 (RIP) 和双路西法酶记者测定.
主要成果:
- 在HCC组织和细胞中,miR-129-2-3p的表达下调.
- miR-129-2-3p的过度表达抑制了HCC细胞的增殖,入侵,迁移和上皮-介质细胞过渡 (EMT).
- 在HCC中,SEMA4C被上调,其降低抑制了HCC的进展;SEMA4C过度表达逆转了miR-129-2-3p的影响.
结论:
- 在HCC中,miR-129-2-3p充当瘤抑制剂.
- miR-129-2-3p通过直接准SEMA4C.p,可以抑制HCC的进展.
- 这种miRNA-mRNA相互作用为HCC提供了潜在的治疗策略.
相关概念视频
02:23Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
02:23Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
01:06Induced Pluripotent Stem Cells
4.0K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
4.0K
01:19Cadherins in Tissue Organization
3.0K
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
Cell Sorting During Development
Cell sorting plays an...
3.0K
02:33Hedgehog Signaling Pathway
7.3K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.3K
01:22Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.2K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K