相关实验视频

Updated: Jun 20, 2025

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Published on: August 12, 2019

通过目标丰富测序试验,解开Hordeum物种基因树不一致的来源

Yanlong Jin¹, Xin Du², Congcong Jiang³

¹State Key Laboratory of Crop Gene Resources and Breeding, Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization (MARA), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest AandF University, Yangling 712100, China.

Molecular phylogenetics and evolution

|July 17, 2024

概括

研究人员开发了新的基因型测试试验,以解开Hordeum物种的进化历史. 不完整的血统排序和内进事件被确定为影响它们复杂的家族遗传关系的关键因素.

科学领域:

植物基因组学和进化生物学
农业科学和作物改良.

背景情况:

Hordeum 是 Triticeae 部落中的一个重要基因,包括 33 种不同的物种和 4 个子基因组.
野生Hordeum物种作为改善大麦和小麦的关键遗传资源.
了解Hordeum复杂的进化历史对于未来的育种计划至关重要.

研究的目的:

开发一个强大的管道,用于对Hordeum物种的基因型和遗传学分析.
为了研究进化关系和因素,有助于 Hordeum 属内的遗传学不一致.
评估对Hordeum和相关的Triticeae物种的新型目标丰富试验的有用性.

主要方法:

开发和应用两种新的目标增强试验 (HorCap v1.0和BarPlex v1.0).
通过测试捕获的全基因组分布的片段的高通量测序.
来自Hordeum,Triticum和Aegilops属的40个物种的82个样本的基因定型.
遗传学分析包括祖先状态重建和父母捐赠者的推断.

主要成果:

无论是HorCap v1.0还是BarPlex v1.0测试都显示出高效的基因型定型能力.
两种测试的整合显著提高了Hordeum家族遗传树的强度和分辨率.
不完整的血统分类 (ILS) 被确定为四个亚基因组中遗传学不一致的主要驱动因素.
在新世界Hordeum物种 (I基因组) 中检测到 post-speciation 内进事件.

结论:

开发的试验有效用于Hordeum和其他Triticeae野生物种的基因型和遗传学分析.
ILS和内进化是塑造黄蜂群进化的重要因素,需要修订后遗传学理解.
这项研究提供了对Hordeum系谱的新见解,为改善作物提供了宝贵的资源.

关键词:

基因树的不一致性荷尔代姆 (Hordeum) 是一个巨大的群体.人类的家谱 (Phylogeny) 是一个学科.聚类物种的多种化.目标丰富的测序是指目标丰富的测序.三叶植物 (Triticeae) 是一种三叶植物.

更多相关视频

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

相关概念视频

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

Gene Evolution - Fast or Slow?

Gene Evolution - Fast or Slow?

The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...

Gene Duplication and Divergence

Gene Duplication and Divergence

The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...