马洛尼尔-CoA减少酶的C端域的动态表征
Mirela Tkalcic Cavuzic1, Amanda Silva de Sousa2, Jeremy R Lohman2
1Department of Biological Sciences, Louisiana State University; Baton Rouge, LA 70803, USA.
Biochimica et biophysica acta. Proteins and proteomics
|July 17, 2024
概括
马洛尼尔-CoA还原酶可以减少.
科学领域:
- 生物化学 生物化学
- 酶学 是一种酶学.
- 代谢工程是代谢工程.
背景情况:
- 马洛尼尔-CoA还原酶 (MCR) 是 *Chloroflexus aurantiacus* 的碳固定途径中的关键.
- MCR将马洛尼尔-CoA转化为3基酸 (3HP),这是一个工业化学前体.
- 该酶有两个独立活跃的域:C-终端用于马洛尼尔-CoA降解和N-终端用于阿尔代降解.
研究的目的:
- 为了动态地表征马洛尼尔-CoA还原酶的C终端域.
- 阐明反应机制并确定关键的催化残留物.
- 为可持续发展的3HP生物合成的发展提供信息.
主要方法:
- 酶动力学 (初始速度,抑制研究).
- 对基质类似物和动态同位素效应的分析.
- 用pH值速率配置文件来确定催化残留物pKa.
主要成果:
- C终端域遵循一个有序的动力机制,NADPH首先结合,其次是马洛尼尔-CoA.
- 首先释放的是马龙半化物,其次是CoA和NADP+释放.
- 具有pKa ~ 8.8的残留物需要质子来产生活性,而化物转移涉及NADPH的pro-S.
结论:
- C端域的机制是阶段性的和有序的,产品释放是部分限制速度的.
- 了解这种机制对于工程MCR对于高效的3HP生产至关重要.
- 这项研究支持开发3HP的环保生物合成,用于工业应用.
相关概念视频
01:46Electron Transport Chain: Complex I and II
12.7K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
12.7K
02:36The Citric Acid Cycle
151.2K
The citric acid cycle, also known as the Krebs cycle or TCA cycle, consists of several energy-generating reactions that yield one ATP molecule, three NADH molecules, one FADH2 molecule, and two CO2 molecules.
151.2K
01:28Overview of Fatty Acid Metabolism
30.4K
Lipids also are sources of energy that power cellular processes. Like carbohydrates, lipids are composed of carbon, hydrogen, and oxygen, but these atoms are arranged differently. Most lipids are nonpolar and hydrophobic. Major types include fats and oils, waxes, phospholipids, and steroids.
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
30.4K