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Updated: Jun 20, 2025

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
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克里斯普尔-Cas12a表现出金属依赖的特异性切换

Giang T Nguyen1, Michael A Schelling1, Akshara Raju1

  • 1Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, USA.

Nucleic acids research
|July 17, 2024
PubMed
概括

基因编辑工具CRISPR-Cas12a的特异性随着离子度的变化而变化. 降低Mg2+增强了对某些目标的结合,但增加了来自其他不匹配的错误,影响了其在生物技术中的使用.

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