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一个决定性,c-di-GMP-依赖的程序确保在细胞运动过程中生成表型相似,对称的子细胞
María Pérez-Burgos1, Marco Herfurth1, Andreas Kaczmarczyk2
1Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
Nature communications
|July 17, 2024
概括
在细胞分裂过程中,Myxococcus xanthus使用循环迪加尼酸单酸盐 (c-di-GMP) 程序,以确保子细胞在表型上相似. 这种机制涉及DmxA酶,保证了新细菌细胞的一致的运动行为.
科学领域:
- 细菌细胞的分裂是细菌细胞的分裂.
- 微生物生理学 微生物生理学
- 分子微生物学分子微生物学
背景情况:
- 细菌的表型异质性来自于随机性或决定性程序.
- 确定性程序经常使用循环迪加尼酸单酸盐 (c-di-GMP) 来产生细胞间的变异.
- 减少细菌表型异质性的机制不太了解.
研究的目的:
- 在Myxococcus xanthus中识别一个确定性的程序,以尽量减少现象型异质性.
- 阐明周期性迪加尼酸单酸盐 (c-di-GMP) 在确保表型相似的子细胞中的作用.
主要方法:
- 研究的Myxococcus xanthus突变体缺乏迪加尼酸环酶DmxA.
- 在细胞分裂过程中分析了DmxA的局部和活性.
- 评估DmxA对周期性迪加尼酸单酸盐 (c-di-GMP) 水平和运动性蛋白质分布的影响.
主要成果:
- 缺乏DmxA的Myxococcus xanthus细胞表现出异常的运动性.
- DmxA被招募到细胞分裂部位,并在细胞动力学过程中被激活,暂时增加c-di-GMP水平.
- 这种c-di-GMP增加确保了运动成分的对称分布,产生类似的子细胞.
结论:
- 一个依赖于c-di-GMP的程序,涉及DmxA,最大限度地减少了Myxococcus xanthus.的表型异质性.
- 细菌可以通过在分裂期间的不对称酶局部化来控制子细胞的相似性或不相似性.
- 这项研究揭示了一种维持细菌表型一致性的机制.
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