相关实验视频

Updated: Jun 20, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

在Aquilaria yunnanensis的染色体层次的基因组组合

Meifei Li¹, Yingmin Zhang², Yi Wang¹

¹School of Life Sciences, Yunnan Normal University, Kunming, 650500, China.

Scientific data

|July 17, 2024

概括

临灭绝的木树 (Aquilaria yunnanensis) 现在拥有染色体水平的参考基因组. 这种基因组资源有助于保护工作和对这种有价值的药用植物的理解.

科学领域:

基因组学就是基因组学.
保护生物学保护生物学
植物科学植物科学

背景情况:

云南 (Aquilaria yunnanensis) 是一个临灭绝的,生产木的树种.
阿加尔木具有重要的药用和经济价值.
人口下降需要基因组数据来保护.

研究的目的:

组装Aquilaria yunnanensis的染色体水平参考基因组.
为保护和研究这个危物种提供基因组资源.

主要方法:

使用了BGI短读,PacBio HiFi长读,以及Hi-C技术.
组装了一个847.04 Mb的基因组,N50为99.68 Mb.
在八个伪染色体上定了805.49 Mb,确定了两个没有间隙的染色体.

主要成果:

成功生成了高质量的染色体级基因组组.
标注 27,955个蛋白质编码基因和74.65%的重复元素.
该组合包括八个伪染色体,其中两个是无间隙的.

结论:

染色体水平的基因组为Aquilaria yunnanensis的保护提供了宝贵的资源.
促进功能基因组学,分子育种和进化研究的未来研究.
支持对木形成和 Aquilaria 种类演变的理解.

更多相关视频

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

2D and 3D Chromosome Painting in Malaria Mosquitoes

2D and 3D Chromosome Painting in Malaria Mosquitoes

Published on: January 6, 2014

相关概念视频

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

Lampbrush Chromosomes

Lampbrush Chromosomes

In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...