牛血液转录组的内分析以确定lncRNA及其在牛结核病期间的作用
Priyanka Garg1, Venkata Krishna Vanamamalai1,2, Shailesh Sharma3,4
1National Institute of Animal Biotechnology (NIAB), Opp. Journalist Colony, Near Gowlidoddi, Extended Q City Road, Gachibowli, Hyderabad, Telangana, 500032, India.
Scientific reports
|July 17, 2024
概括
长非编码RNAs (lncRNAs) 调节基因表达,并在牛结核免疫力中发挥作用. 这项研究确定了牛中涉及的关键 lncRNA 和基因.
科学领域:
- 基因组学就是基因组学.
- 免疫学 免疫学 免疫学
- 生物信息学是一种生物信息学.
背景情况:
- 长非编码RNAs (lncRNAs) 是关键的基因调节剂,但它们在牛结核病 (bTB) 免疫力中的作用尚未得到研究.
- 由Mycobacterium bovis (M. bovis) 引起的牛结核病对牛的健康和全球粮食安全构成重大威胁.
研究的目的:
- 调查 lncRNAs 在宿主免疫反应中对M. bovis感染在牛中的作用.
- 确定与M. bovis感染相关的差异表达基因 (DEGs) 和 lncRNAs (DElncRNAs).
主要方法:
- 在M. bovis感染和未感染的小牛的公开可用的转录组数据的in-silico分析.
- 识别和定量 lncRNA 和基因.
- 不同表达分析,基因本体学 (GO) 丰富,通路分析 (Reactome) 和协同表达分析.
主要成果:
- 总共有51,812个lncRNAs被确定.
- 在感染和时间点之间,有216个基因和260个lncRNAs被差异地表达.
- 与免疫相关的GOs和途径被DEGs丰富,同时表达分析将DElncRNA与这些免疫基因联系起来.
结论:
- 在M. bovis感染期间,lncRNAs受到动态调节,并参与牛的免疫反应.
- 已识别的基因-lncRNA相互作用为宿主-病原体动态提供了洞察力,并为牛的遗传改进提供了潜在的目标.
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