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相关概念视频

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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相关实验视频

Updated: May 8, 2026

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
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In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

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在体内,单细胞CRISPR揭示了瘤进化中的特异性TNF程序

Peter F Renz1, Umesh Ghoshdastider1, Simona Baghai Sain2

  • 1Institute for Regenerative Medicine (IREM), University of Zurich, Schlieren-Zurich, Switzerland.

Nature
|July 17, 2024
PubMed
概括

这项研究揭示了瘤死因 (TNF) 在癌症演变过程中的不同信号程序. 一个新的体内CRISPR屏幕识别了TNF信号作为状细胞癌的克隆扩张和入侵的驱动因素.

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Last Updated: May 8, 2026

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

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科学领域:

  • 癌症生物学
  • 遗传学
  • 免疫学

背景情况:

  • 瘤生成涉及由突变驱动的克隆扩张,但导致恶性转变的机制尚不清楚.
  • 了解正常组织中的克隆动态对于破译早期癌症发展至关重要.

研究的目的:

  • 在体内使用单细胞CRISPR系统地研究瘤进化过程中的全组织克隆动态和基因程序.
  • 发现导致细胞癌扩散和恶性转变的机制.

主要方法:

  • 开发了一种体内单细胞CRISPR策略与超声波导向的子宫内微注射.
  • 通过单细胞RNA测序和指导捕获利用纵向监测来分析克隆扩张.
  • 研究了150个经常发生突变的状细胞癌基因的作用.

主要成果:

  • 鉴定了一种瘤亡因子 (TNF) 信号模块,涉及TNF受体1和巨细胞,作为表皮组织中克隆扩张的驱动因素.
  • 在瘤形成过程中观察到TNF信号模块的下调,转换为侵袭性癌细胞的TNF程序.
  • 证明自性TNF程序调解侵袭性质,并与患者的生存时间缩短相关.

结论:

  • 在体内单细胞CRISPR查是研究哺乳动物组织动态的强大工具.
  • 独特的TNF信号程序在瘤进化,克隆扩张和入侵中发挥着关键作用.
  • 了解克隆扩张和瘤发生之间的相互作用对于癌症研究至关重要.