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Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
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具有基础膜组件的创新基质设计,用于增强内皮细胞功能和内皮化.

Yuriy Snyder1, Soumen Jana1

  • 1Department of Chemical and Biomedical Engineering, University of Missouri, 1406 Rollins Street, Columbia, MO, 65211, USA.

Advanced healthcare materials
|July 18, 2024
PubMed
概括

在纤维基板上创建人工底层膜显著改善了内皮细胞的生长和功能. 这种方法通过促进更好的内皮质化和降低血栓形成风险来提高血管移植的性能.

科学领域:

  • 生物材料科学 生物材料科学
  • 血管生物学 血管生物学
  • 组织工程是组织工程.

背景情况:

  • 脱细胞化的血管移植在加工过程中往往会失去关键的底层膜蛋白质,阻碍内皮细胞的粘附和功能.
  • 底层膜组件的损失,如拉米林和IV原,可以导致内皮质形成不良,血栓形成和内皮增生.
  • 目前的策略很难完全复制本地底层膜的亲内皮质性质.

研究的目的:

  • 开发一种新的仿生方法来增强血管移植基板上的内皮细胞生长.
  • 为了创建一个人造的底层膜基板,保留必要的内皮细胞促进蛋白质.
  • 评估这种工程基质在支持内皮细胞增殖和功能的有效性.

主要方法:

  • 纤维细胞在纤维基质上培养,以产生细胞外矩阵膜基质 (EMMS).
  • 该EMMS经过了抗原去除 (AR) 处理,产生了一种AR处理的膜基板 (AMS).
  • 人体内皮细胞在AMS上进行培养,并与脱细胞化大动脉组织上的细胞进行比较.

主要成果:

  • 在AMS上培养的内皮细胞显示出增强的增殖和氧化生产.
  • AMS促进了静止/恒温,自和抗血栓因子的内皮标记物的表达增加.
  • 与非细胞化的大动脉组织相比,工程基板表现出优越的性能.
关键词:
聚氨是一种聚氨.抗原的去除,抗原的去除.脱细胞化 脱细胞化内皮细胞是内皮细胞.内皮质化 (endothelialization) 是一种内皮质化的过程.纤维细胞的细胞.血管移植是一种血管移植.

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结论:

  • 预先用仿生基底膜赋予纤维基底是一种改善内皮质化的有希望的策略.
  • 这种方法为血管移植应用的脱细胞化组织的局限性提供了潜在的解决方案.
  • 开发的AMS支持一种更生理的内皮细胞表型,这对于预防移植失败至关重要.