相关实验视频
Updated: Jun 20, 2025

09:33
Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules
Published on: May 12, 2017
14.6K
林28a与Igf2bp3形成RNA结合复合体,以调节肌肉干细胞的压力颗粒中的A-修饰应激反应基因
Dan Song1,2, Yu Chen1,2,3, Peng Wang1,2,3
1Key Laboratory of Organ Regeneration and Reconstruction, State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Cell proliferation
|July 18, 2024
概括
林28同源A (Lin28a) 和Igf2bp3蛋白稳定肌肉干细胞中的DNA修复基因mRNA. 这种机制增强了DNA修复,并促进了干细胞自我更新.
科学领域:
- 分子生物学分子生物学
- 干细胞生物学 干细胞生物学
- 在RNA代谢过程中.
背景情况:
- 林28同源A (Lin28a) 是一种RNA结合蛋白,在早期发育中至关重要,随着成熟而下降.
- 林28a保持成人肌肉干细胞 (MuSCs) 在胚胎状的状态,但其调节机制尚未完全理解.
- 在肌肉组织中,Lin28a的表达特别高.
研究的目的:
- 阐明Lin28a在成年肌肉干细胞中的RNA代谢调节机制.
- 调查Lin28a和Igf2bp3在MuSC自我更新和DNA修复中的协作作用.
- 探索对再生医学的潜在影响.
主要方法:
- 对组织特异表达的BioGPS分析.
- 通过流细胞计分类Lin28a阳性MuSCs (Lin28a+ MuSCs).
- 针对Lin28a,Igf2bp3和m6A的RNA免疫沉降测序 (RIP-seq) 进行测序.
- 同免疫沉和免疫光分析.
主要成果:
- 林28a+ MuSCs 显示扩散率有所增加.
- Lin28a结合的转录在DNA修复,细胞循环和应激反应途径中得到丰富.
- 发现Lin28a和Igf2bp3在应力颗粒 (SGs) 中同局部化,m6A的修饰促进了SG的形成.
- 林28a和Igf2bp3共同调节DNA修复基因 (例如Fancm,Usp1) 并稳定它们的mRNA.
- 林28a稳定Igf2bp3,Usp1和FancmmmRNAs,增强DNA修复并促进MuSC的自我更新.
结论:
- 林28a和Igf2bp3通过mRNA稳定和增强DNA修复,在维持MuSC功能方面发挥着关键作用.
- 林28a-Igf2bp3复合体通过加强DNA修复机制来调节MuSC的自我更新,以应对压力.
- 了解这些途径为干细胞生物学和潜在的再生疗法提供了洞察力.
相关概念视频
Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
Regulation of Expression at Multiple Steps
886
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
886
Regulation of the Unfolded Protein Response
2.4K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.4K
RNA Stability
33.5K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.5K
Regulation of Expression Occurs at Multiple Steps
22.6K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.6K
Riboswitches
8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K

