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缺乏C5aR2会减少中性粒细胞和巨细胞中C5aR1的分布和表达

Ting Zhang^1,2, Ning Ma², Jiaxing Wang³

¹Department of Pathology The Second Affiliated Hospital School of Medicine Xi'an Jiaotong University, Xi'an 710004, China.

Journal of immunology research

|July 18, 2024

概括

补充成分5a受体2 (C5aR2) 缺乏会降低中性粒细胞和巨细胞中C5aR1的表达. 这表明C5aR2在调节C5aR1功能方面发挥着作用.

科学领域:

免疫学免疫学免疫学
补充系统补充系统
具有天生的免疫力.

背景情况:

补充激活产品C5a与C5a受体1 (C5aR1) 和C5a受体2 (C5aR2) 相结合.
C5aR2在调节C5aR1信号中的作用是复杂的,正在研究中.
之前的研究表明C5aR1和C5aR2在高C5a度下可能形成异构体.

研究的目的:

研究C5aR2缺乏对中性粒细胞和巨细胞C5aR1表达的影响.
在正常和炎症条件下探索C5aR2在先天性免疫细胞功能中的作用.
为了确定C5aR2淘汰是否影响WT和C5ar2淘汰小鼠中的C5aR1转录.

主要方法:

在C57BL/6背景上使用了野生型 (WT) 和C5ar2淘汰赛 (KO) 鼠标.
分析了脏,肝脏,骨髓,周围血液白细胞和腹巨细胞中的C5aR1mRNA和蛋白质水平.
采用流细胞计 (FACS) 来检测中性粒细胞和巨细胞的表面C5aR1表达.
诱导腹膜炎使用硫糖酸盐注射来模拟炎症.

主要成果:

在正常组织中,WT和C5ar2KO小鼠的C5aR1mRNA水平与正常组织中的WT和C5ar2KO小鼠相似.
从C5ar2KO小鼠的循环中性粒细胞上,C5aR1表面表达减少.
在C5ar2KO小鼠中,硫糖酸诱导的腹膜炎显示中性粒细胞的招募增加.
在炎症后,C5ar2 KO小鼠的巨细胞中,C5aR1 mRNA和蛋白质的表达减少.

结论:

C5aR2缺乏导致中性粒细胞和巨细胞中C5aR1分布和表达的减少.
在中性粒细胞化学反应中,C5aR2似乎对抗C5aR1信号传递.
C5aR2可能与C5aR1合作,调节巨细胞中的炎症信号.
在中性粒细胞和巨细胞中,C5aR2表现出与C5aR1调节有关的差异性功能.

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