在IVM系统中补充NPPC和AREG会改变牛肉COC的mRNA翻译和衰变程序相关的基因表达
Helena Fabiana Reis de Almeida Saraiva1, Juliano Rodrigues Sangalli1, Luana Alves1
1Faculdade de Zootecnia e Engenharia de Alimentos, Departamento de Medicina Veterinária, Universidade de São Paulo, Pirassununga, SP, Brasil.
Animal reproduction
|July 18, 2024
概括
在卵细胞成熟期间,累积细胞对NPPC和AREG补充剂的反应更强,影响mRNA转化和衰变. 卵细胞显示的变化较少,影响了早期胚胎发育.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 发展生物学 发展生物学
背景情况:
- 卵细胞成熟涉及协调的mRNA转化和衰变,以成功的胚胎发育.
- 在mRNA翻译 (CPEB1,CPEB4) 和降解 (CNOT7,ZFP36L2) 中的关键基因对于这个过程至关重要.
- 与体内对应物相比,在体外成熟的牛卵细胞中发现ZFP36L2的下调.
研究的目的:
- 研究NPPC和AREG补充剂对牛卵细胞和细胞在体外成熟 (IVM) 过程中的mRNA翻译和降解途径的影响.
- 评估卵细胞和累积细胞对这些调节器的差异反应.
- 了解对母亲mRNA调制的影响,并改善IVM结果.
主要方法:
- 研究了翻译和降解机制基因的mRNA水平 (CPEB1,CPEB4,CNOT7,ZFP36L2).
- 使用了NPPC的IVM前步骤和AREG的修改IVM.
- 在不同IVM时间点 (9h和19h) 分析了卵细胞和累积细胞中的基因表达.
主要成果:
- NPPC和AREG补充剂在IVM的9小时内大规模上调了参与累积细胞转化和衰变过程的基因.
- 卵细胞对NPPC和AREG的反应较小;ZFP36L2在9h和19hIVM下调,但只有一个位基因 (KDM4C) 显示出差异性表达.
- 累积细胞表现出对NPPC和AREG补充剂对转化和mRNA衰变程序的反应更强的倾向.
结论:
- 累积细胞在体外成熟过程中对NPPC和AREG补充剂的反应比卵细胞更强,有关mRNA转化和衰变.
- 这些发现凸显了积分细胞在调节母体mRNA调节中的关键作用.
- 进一步的研究可以通过利用这些调节器来优化IVM协议,以增强胚胎发育.
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