原度调节中性粒细胞外流和迁移,以内皮依赖的方式响应感染
Christopher J Calo1, Tanvi Patil1, Mallory Palizzi1
1Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO, United States.
Frontiers in immunology
|July 18, 2024
概括
中性粒细胞向感染部位的迁移取决于细胞外基质. 较高的原度减少了中性粒细胞的运动,这种影响取决于内皮细胞,突出显示了微环境在免疫反应中的作用.
科学领域:
- 免疫学 免疫学 免疫学
- 生物材料科学 生物材料科学
- 微流体学 微流体学
背景情况:
- 中性粒细胞对抗感染至关重要,但它们的失调有助于疾病.
- 细胞外基质 (ECM) 组成的变化与影响中性粒细胞的疾病有关.
- 对于ECM蛋白组成对中性粒细胞对感染反应的影响尚未完全理解.
研究的目的:
- 为了研究细胞外矩阵组成如何影响中性粒细胞在感染期间的反应.
- 确定原度在中性粒细胞外流和迁移中的作用.
主要方法:
- 使用了芯片上的感染微流体装置,模仿血管内皮和ECM.
- 人的静脉内皮细胞在不同度的I型原蛋白水凝 (2,4,6毫克/毫升) 上培养.
- 通过*Pseudomonas aeruginosa**引入并刺激人类初级中性粒细胞.
主要成果:
- 原度没有影响内皮细胞密度或屏障功能.
- 中性粒细胞扩散在4mg/mL原体凝中高于6mg/mL凝.
- 中性粒细胞迁移速度和距离在2 mg/mL凝中最高,并且随着原度的增加而下降,这种影响取决于内皮细胞.
结论:
- 中性粒细胞的扩散和迁移是由内皮细胞依赖的原度调节的.
- 这项研究表明,组织微环境机制和结构如何影响中性粒细胞对感染的反应.
- 微生理系统是研究中性粒细胞调节的宝贵工具.
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