线场动态光学连贯性断层扫描平台用于生物组织的体积评估
Keyu Chen1, Stephanie Swanson1, Kostadinka Bizheva1,2,3
1Department of Physics and Astronomy, University of Waterloo, Waterloo N2 L 3G1, ON, Canada.
Biomedical optics express
|July 18, 2024
概括
我们开发了一种新的高速光谱域线场dOCT (SD-LF-dOCT) 系统,用于快速的生物组织体积成像. 这种先进的动态光学相干断层扫描能够详细可视化细胞结构和运动性.
科学领域:
- 生物医学光学 生物医学光学
- 光学成像技术的成像
- 细胞生物学 细胞生物学
背景情况:
- 动态光学相干断层扫描 (dOCT) 通过分析依赖时间的信号波动来增强OCT图像的对比度.
- 之前的dOCT研究仅限于2D成像,因为采集率低.
- 获取速度的限制阻碍了生物组织的体积dOCT.
研究的目的:
- 引入一种新的高速光谱域直线场dOCT (SD-LF-dOCT) 系统.
- 实现生物组织的快速体积dOCT成像.
- 为各种生物医学应用提供可适应的成像参数.
主要方法:
- 开发了一种具有高采集速度 (2,000 fps) 的光谱域线场dOCT系统.
- 使用可更换的焦点镜头对进行可调的横向分辨率 (1.16.4μm) 和视野 (250×250μm2至1.4×1.4mm2).
- 实现了约1.9微米的轴分辨率和90.5dB的最大灵敏度.
主要成果:
- 证明了植物 (黄瓜),动物 (老鼠肝脏) 和人类前列腺癌球体的体积dOCT成像.
- 成功地在3D中可视化了细胞和亚细胞结构.
- 启用了组织内细胞运动率的评估.
结论:
- SD-LF-dOCT系统克服了dOCT之前的速度和维度限制.
- 这项技术促进了生物组织的高分辨率体积可视化和功能评估.
- 该系统的适应性使其适用于广泛的生物医学研究.
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