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胎盘衍生的介质干细胞通过外体蛋白相互作用网络促进糖尿病伤口愈合

Cheng Peng¹, Hongbo Xu², Quan Zhuang³

¹Department of Burns and Plastic Surgery, The Third Xiangya Hospital of Central South University, Changsha, China.

Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society

|July 18, 2024

概括

人类胎盘衍生的介质干细胞 (plaMSC) 和它们的外体通过增强细胞迁移和增殖,促进糖尿病伤口愈合. 蛋白质组学揭示了参与组织再生和重塑的关键蛋白质,提供了潜在的治疗策略.

科学领域:

再生医学是一种再生医学.
细胞生物学细胞生物学
生物化学生物化学

背景情况:

糖尿病耐火性伤口缺乏有效的治疗方法,这构成了重大的临床挑战.
人类胎盘衍生的介质干细胞 (plaMSCs) 在伤口愈合方面表现有前途.
来自plaMSCs (plaMSCs-Ex) 的外体是它们治疗效果的关键调解者.

研究的目的:

为了研究plaMSCs和plaMSCs-Ex.的蛋白质含量.
阐明plaMSCs-Ex增强糖尿病伤口愈合的机制.
确定参与plaMSCs-Ex.的治疗作用的关键蛋白质和途径.

主要方法:

使用无标签蛋白质组学分析了plaMSCs和plaMSCs-Ex的蛋白质组成.
进行了生物信息学分析,包括基因本体学 (GO) 注释,KEGG通路丰富,基因组丰富分析 (GSEA) 和蛋白质与蛋白质相互作用 (PPI) 分析.
在 plaMSCs-Ex 中分别表达的蛋白质被确定并进行了表征.

主要成果:

在plaMSCs-Ex中丰富的蛋白质与细胞外矩阵组织,上皮形态发生,细胞生长,粘附,增殖和血管生成显著相关.
PPI分析发现了两个与伤口愈合相关的集群,其中包括POSTN,FN1,SPARC,TIMP1,SERPINE1,LRP1和原体的枢纽蛋白.
蛋白质组概况表明plaMSCs-Ex.的再生和组织重塑功能多样化.

结论:

来自人类胎盘介质干细胞 (plaMSCs-Ex) 的外体具有丰富的蛋白质组,有助于糖尿病伤口愈合.
这些外体蛋白质参与了诸如细胞增殖,迁移和组织重塑等关键过程.
plaMSCs-Ex代表了一种有前途的无细胞治疗策略,用于治疗糖尿病耐火性伤口.

关键词:

凯格 (KEGG) 是一个这是一个PPI PPI.糖尿病的伤口外基因组是外基因组中的一个.没有标签的蛋白质组学.胎盘衍生中介细胞干细胞.

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