相关实验视频
Updated: Jun 20, 2025

04:39
Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model
Published on: March 17, 2023
1.6K
在哈西莫托甲状腺炎中甲状腺功能障碍:关于miR-29a和TGFβ1的假定作用的试点研究
Maria Consiglia Trotta1, Daniela Esposito2,3, Raffaela Carotenuto4
1Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", Naples, Italy.
Endocrine
|July 18, 2024
概括
患有甲状腺功能低下症的哈西莫托甲状腺炎患者表现出较低的miR-29a和较高的TGFβ1.1. 在这些患者中,Levothyroxine治疗可能会恢复miR-29a水平.
科学领域:
- 内分泌学 在内分泌学.
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 哈西莫托甲状腺炎 (HT) 是一种常见的自身免疫性疾病,导致甲状腺功能障碍.
- 背后的HT病原体的精确分子机制仍然不完全理解.
- 微RNA (miRNA) 和生长因子与自身免疫性甲状腺疾病有关.
研究的目的:
- 在患有哈希莫托氏甲状腺炎的患者中研究miRNA-29a (miR-29a) 和转化生长因子β1 (TGFβ1) 的血清水平.
- 为了将这些水平与不同类型的甲状腺功能状况相关联:乙甲状腺 (Eu-HT),未经治疗的甲状腺功能低下症 (Hypo-HT) 和治疗的甲状腺功能低下症 (sub-HT).
主要方法:
- 分析了29名HT患者 (13 Eu-HT, 8 Hypo-HT, 8 sub-HT) 的血清样本.
- 使用定量逆转录聚合酶连锁反应 (qRT-PCR) 来量化血清miR-29a水平.
- 使用酶相关免疫吸收试验 (ELISA) 测量血清TGFβ1水平.
主要成果:
- 与Eu-HT和亚HT组相比,Hypo-HT患者的血清miR-29a水平显著降低.
- 血清TGFβ1水平在Hypo-HT患者显著升高,与Eu-HT和亚HT组相比.
- 在血清miR-29a和TSH水平之间,以及在血清miR-29a和TGFβ1水平之间发现了显著的负相关性.
结论:
- 在HT中未治疗的甲状腺功能低下与miR-29a降低和TGFβ1.1.增加有关.
- 在HT患者中,Levothyroxine替代疗法可能会导致恢复miR-29a水平.
- 这些发现表明miR-29a和TGFβ1在HT病理生理学和治疗反应中的潜在作用.
相关概念视频
Synthesis and Regulation of Thyroid Hormones
4.5K
Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...
4.5K
TGF - β Signaling Pathway
7.3K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.3K
Functions of Thyroid Hormones
2.7K
The thyroid hormone (TH) plays a pivotal role in the intricate orchestration of physiological processes, exerting profound effects on development, metabolism, and homeostasis throughout different life stages.
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...
2.7K
The Thyroid Gland
3.8K
The thyroid gland is a small, butterfly-shaped gland located in the neck and covers the anterior surface of the trachea. The gland has two lateral lobes connected by a thin tissue mass called the isthmus. Internally, each lobe comprises many small spherical structures known as thyroid follicles, surrounded by a network of blood vessels.
The follicles have a central cavity lined by simple cuboidal to squamous epithelial cells called follicular cells. These cells produce the glycoprotein...
The follicles have a central cavity lined by simple cuboidal to squamous epithelial cells called follicular cells. These cells produce the glycoprotein...
3.8K
Major Hormones and Their Functions
364
Hormones, the biochemical messengers produced by endocrine glands, are pivotal in regulating bodily functions and maintaining homeostasis. Each hormone's balance is crucial; imbalances can lead to significant physiological disruptions. Major hormones include oxytocin, cortisol, epinephrine, estrogen, testosterone, thyroxine, growth hormone, insulin, and glucagon.
Oxytocin, produced in the hypothalamus and released by the pituitary gland, plays a role in social bonding, childbirth, and...
Oxytocin, produced in the hypothalamus and released by the pituitary gland, plays a role in social bonding, childbirth, and...
364
T Cell Types and Functions
990
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
990

