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多尺度光催化近距离标记揭示了细胞表面上的和细胞之间的邻居

Zhi Lin¹, Kaitlin Schaefer¹, Irene Lui¹

¹Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA.

Science (New York, N.Y.)

|July 18, 2024

概括

这项研究引入了一种使用Eosin Y和可见光绘制蛋白相互作用的多功能近距离标记蛋白质组学 (PLP) 方法. 该平台揭示了新的细胞表面蛋白质网络和潜在的免疫治疗点.

科学领域:

生物化学
蛋白质组学
细胞生物学

背景情况:

近距离标记蛋白质组学 (PLP) 提供了对细胞内的蛋白质相互作用的见解.
现有的PLP方法在分辨率和范围方面存在局限性.

研究的目的:

开发一种可调节分辨率的多尺度PLP方法,用于绘制蛋白质社区.
分析瘤性表皮生长因子受体 (EGFR) 和细胞-细胞突触的蛋白相互作用.

主要方法:

使用商用光催化剂Eosin Y,通过可见光激活.
使用具有不同标记半径的光探测器进行多尺度分析.
使用免疫测试和AlphaFold-Multimer预测验证的邻近蛋白质.

主要成果:

成功分析了EGFR的蛋白邻居,确定了20多个验证的邻居.
由T细胞参与者和CAR T细胞诱导的细胞-细胞突触中的蛋白质网络.
展示了该平台在细胞表面和细胞之间绘制局部和远端蛋白质网络的能力.

结论:

集成的多尺度PLP平台为细胞表面互动图绘制提供了强大的工具.
这种方法有助于识别横向信号合作伙伴和潜在的免疫治疗点.
该方法为各种生物研究提供可调节的分辨率.

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