转录因子AbrB调节ROS生成和清除在Bacillus licheniformis中的作用
Penghui He1, Shiying Hu1, Yongjia Zhang1
1State Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, College of Life Sciences, Hubei University, Wuhan 430062, China.
Microbiological research
|July 18, 2024
概括
转录因子AbrB控制了Bacillus licheniformis中的反应性氧物种 (ROS). 它的无活化通过减少ROS生成和增强抗氧化防御来增强压力下的细胞生存.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 活性氧物种 (ROS) 的氧化损伤阻碍了微生物在压力下生长和发酵.
- 细胞的抗氧化能力通常随着微生物生长而增加.
- 了解氧化还原平衡调节对于改善微生物工业应用至关重要.
研究的目的:
- 研究过渡状态转录因子AbrB在调节ROS积累和Bacillus licheniformis的抗氧化能力中的作用.
- 阐明AbrB控制的有关ROS生成和清除途径的监管网络.
主要方法:
- 基因淘汰实验,以评估AbrB删除对ROS水平和细胞存活的影响.
- 转录组测序分析以确定参与ROS代谢的AbrB调节基因.
- 对与氧化酶, siderophores,铁化剂和抗氧化酶相关的基因表达的分析.
主要成果:
- 在过氧化压力下,AbrB淘汰显著降低了23.91%的细胞内ROS积累,并增加了1.77倍的细胞存活率.
- AbrB通过促进氧化酶和 siderophore 合成,同时抑制铁化剂来调节 ROS 的生成.
- AbrB通过降低关键抗氧化酶 (超氧化脱酶,催化酶,过氧化酶,硫素,硫素减少酶) 和氨酸合成的调节,抑制ROS清除.
结论:
- AbrB作为Bacillus licheniformis细胞内氧化还原平衡的关键调节者,影响ROS生成和清除.
- 在静止阶段AbrB的失活似乎是细胞在进化过程中的自我保护机制.
- 针对AbrB提供了一种潜在的策略,可以提高微生物应激耐受性和发酵性能.
相关概念视频
Bacterial RNA Polymerase
29.4K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
29.4K
Transcription Attenuation in Prokaryotes
15.2K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
15.2K
Prokaryotic Transcriptional Activators and Repressors
20.9K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
20.9K
RNA Polymerase II Accessory Proteins
9.2K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.2K
Types of RNA
63.5K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.5K
Riboswitches
8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K


