一种来自甲类动物的I型溶酶,Pacifastacus leniusculus,作为凝块破坏稳定的酶起作用
Charlotta Ekblom1, Kenneth Söderhäll1, Irene Söderhäll1
1Department of Organismal Biology, Uppsala University, Norbyvägen 18A, 752 36, Uppsala, Sweden.
Fish & shellfish immunology
|July 18, 2024
概括
我们在鱼血细胞中发现了一种新型的无脊椎动物类型的溶酶,它缺乏抗菌muramidase活性. 这种酶Pacifastacus leniusculus i-type lysozyme (Pl-ilys) 具有凝块溶解的特性,这表明它在甲类动物的血液免疫防御中发挥了作用.
科学领域:
- 生物化学 生物化学
- 免疫学 免疫学 免疫学
- 海洋生物学 海洋生物学
背景情况:
- 酶是许多有机体中发现的酶,通常以抗菌muramidase活性而闻名.
- 无脊椎动物类型 (i型) 溶酶,如Destabilase,可以具有其他活动,例如在脊椎动物血块中化特定的键.
- 许多无脊椎动物酶的免疫功能在很大程度上仍然没有表征.
研究的目的:
- 为了识别和表征一种来自淡水的新型i型溶酶,Pacifastacus leniusculus.
- 为了研究这种鱼溶酶的酶活性和潜在的免疫功能,命名为Pl-ilys.
- 为了确定Pl-ilys是否具有muramidase或破坏稳定的活性.
主要方法:
- 在Pacifastacus leniusculus血细胞中识别Pl-ilys,并进行表达分析.
- 使用大肠杆菌表达系统生产复合成熟的Pl-lys.
- 酶定量测试用于测试muramidase和iso-peptidase活性,以及对凝结的鱼蛋白质的凝块溶解活性.
主要成果:
- 在类血细胞和血造组织中发现了一种新型i型溶酶 (Pl-ilys),在注射拉米纳林后升高调节.
- 再组合的Pl-ilys没有显示可检测的muramidase或iso-peptidase活性.
- Pl-ilys在经过转谷氨胺酶处理的鱼凝血蛋白上表现出显著的凝块溶解活性,这表明其功能不稳定.
结论:
- 鉴定到的类溶酶Pl-ilys是一种功能性不稳定酶,而不是一种muramidase.
- Pl-ilys可能在调节血液凝固和潜在的甲类动物的免疫防御中发挥作用.
- 这一发现扩大了已知的无脊椎动物类型溶酶的功能多样性.
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