全基因组识别的VvOFP基因家族和VvOFP4的功能性特征提供了对葡萄中的水果形状的洞察
Yang Dong1, Liyuan Huang1, Jing Liu1
1Sanya Institute of Nanjing Agricultural University, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
International journal of biological macromolecules
|July 18, 2024
概括
葡萄卵子家族蛋白 (OFPs) 通过影响细胞尺寸来调节水果的形状. VvOFP4基因对葡萄果形状的确定至关重要,并为培育多种葡萄品种提供了潜力.
科学领域:
- 植物分子生物学 植物分子生物学
- 遗传学 是一个遗传学.
- 农业科学 农业科学
背景情况:
- 卵巢家族蛋白 (OFPs) 是跨物种果实形状的关键转录调节者.
- 在确定葡萄果形状时OFPs的作用尚不清楚.
研究的目的:
- 调查葡萄OFP基因在果实形状决定中的作用.
- 确定涉及葡萄形态及其对植物生长调节者的反应的特定OFP基因.
主要方法:
- 对16个葡萄OFP基因进行全基因组分析.
- 遗传学和合成分析.
- 基因表达概况在用吉伯酸 (GA3) 和蒂迪亚祖龙 (TDZ) 治疗后.
- 功能性试验涉及烟草和西红中的VvOFP4过度表达.
主要成果:
- 遗传学分析显示,葡萄的VvOFP基因与番茄的OFPs之间存在密切的关系.
- 一些OFP基因的表达,特别是VvOFP4,是由GA3和TDZ治疗调节的.
- 在异质系统中VvOFP4的过度表达改变了植物和生殖器官形态,包括水果形状.
- 过度表达VvOFP4改变了细胞尺寸,影响了器官形态.
- GA3和TDZ处理影响了葡萄的细胞形状,影响了整个水果形态.
结论:
- VvOFP4基因是葡萄果形状的关键调节者,主要通过调节细胞形状来调节.
- VvOFP4为基因改进和育种计划提供了一个有希望的目标,旨在开发具有多种水果形状的葡萄.
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