糖酸酶家族70的前所未有的多样性:对序列,结构和功能的全面分析
Tjaard Pijning1, Lubbert Dijkhuizen2,3
1Biomolecular X-ray Crystallography, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, Groningen 9747 AG, The Netherlands.
Journal of agricultural and food chemistry
|July 18, 2024
概括
葡萄糖酸酸酶家族70 (GH70) 酶合成α-葡萄糖. 这项研究揭示了259个GH70酶中辅助域的广泛结构多样性,为它们的细菌起源和功能提供了新的见解.
科学领域:
- 生物化学 生化学
- 结构生物学 结构生物学
- 微生物学 微生物学
背景情况:
- 糖化酶家族70 (GH70) 包含参与α-葡萄糖合成的细菌细胞外酶.
- 现有的GH70酶的结构数据有限,主要涵盖核心领域,缺乏详细的辅助领域信息.
研究的目的:
- 提供GH70酶的系统概述,详细说明它们的3D结构组织和细菌起源.
- 探索GH70家族中辅助域的结构多样性,并将其与酶功能和起源相关联.
主要方法:
- 生成一个代表性的259个GH70酶集 (234个变,25个非变).
- 酶类型的分析,包括葡萄糖糖酶 (GS),分枝糖酶 (BrS) 和α-葡萄糖转移酶 (α-GT).
- 利用AlphaFold建模来预测和分析所有GH70条目中的3D结构.
主要成果:
- 这项研究包括来自12个细菌家族和3个类的259个GH70酶.
- 分析显示了辅助域的显著结构变化,确定了九种不同的域类型.
- 确定了185个预测的葡萄糖糖酶,15个分支的葡萄糖酶,8个"双胞胎"GS-BrS和51个α-葡萄糖转移酶.
结论:
- 在GH70酶中,辅助域的结构多样性比以前认为的要大.
- 遗传学分布分析表明这些辅助领域的潜在作用.
- 研究了酶特异性,辅助域类型和细菌起源之间的相关性,突出了功能和进化关系.
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