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由垂体衍生的小细胞外囊泡通过其载荷miR-143-3p促进肝脏的修复

Jia-Li Xiong^1,2, Yu-Xuan Wang¹, Jun-Yi Luo¹

¹College of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, 510642, Guangdong, China.

Scientific reports

|July 18, 2024

概括

垂体小细胞外囊泡 (sEVs) 提供miR-143-3p以促进肝脏的修复. 这些sEV增强肝细胞增殖,并通过Wnt/β-catenin通路帮助肝脏再生.

科学领域:

内分泌学在内分泌学.
细胞生物学细胞生物学
肝病学肝病学是一种肝病学.

背景情况:

小型细胞外囊泡 (sEVs) 通过货物转移调解细胞间的通信.
脑下垂体衍生的sEVs在生理条件中的作用仍然不清楚.
肝病是一个重大的全球健康挑战.

研究的目的:

为了研究由下垂体衍生的sEVs对肝功能和修复的影响.
为了确定参与肝脏调节的垂体sEVs中的关键分子成分.
在肝损伤模型中探索垂体sEVs的治疗潜力.

主要方法:

测序和qRT-PCR用于识别垂体sEVs中的miRNA载荷.
产生了miR-143淘汰赛 (KO) 鼠标.
将野生型 (WT) 和miR-143-KO sEVs用于CCl4诱导的肝损伤模型.
对肝脏修复,肝细胞增殖和Wnt/β-catenin通路激活的分析.

主要成果:

鉴定出miR-143-3p是脑垂体sEV中非常丰富的miRNA.
miR-143 KO小鼠表现出降低IGF-1,增加IGFBP5,以及肝细胞生长受损.
下垂体sEVs,特别是WTsEVs,在KO小鼠中显著增强了CCl4损伤后的肝脏修复.
Wnt/β-catenin通路与 pituitary sEVs 的肝脏再生作用有关.

结论:

垂体衍生的sEVs通过其miR-143-3p载荷促进肝细胞的增殖和肝脏的修复.
这项研究阐明了一种新的垂体 - 肝轴调节机制.
下垂体SEVs代表了基于内分泌的肝脏治疗的潜在途径.

关键词:

肝脏肝脏肝脏肝脏下垂体的小细胞外囊泡.核武器的扩散维修维修维修维修在 miR-143-3p 中使用.

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