基因组组装,全长的转录组和红色鱼的异形多样性,Lutjanus argentimaculatus
Mudagandur S Shekhar1, Vinaya Kumar Katneni2, Ashok Kumar Jangam1
1Centre for Bioinformatics, ICAR-Central Institute of Brackishwater Aquaculture, No 75, Santhome High Road, MRC Nagar, Chennai, 600028, Tamil Nadu, India.
Scientific data
|July 18, 2024
概括
研究人员为红树林红 snapper (Lutjanus argentimaculatus) 开发了关键的基因组和转录组资源. 这些基础数据集将推动选择性育种计划和保护这一重要的海洋食物鱼的保护工作.
科学领域:
- 海洋生物学 海洋生物学
- 基因组学就是基因组学.
- 水产养殖科学 水产养殖科学
背景情况:
- 红树林红 (Lutjanus argentimaculatus) 是一种有价值的海洋食物鱼,具有重要的经济和水产养殖重要性.
- 目前,基于基因组选择的L. argentimaculatus的育种计划因缺乏基本的基因组和转录组参考数据而受到阻碍.
研究的目的:
- 为了生成 Lutjanus argentimaculatus. 的高质量的参考基因组组.
- 为L. argentimaculatus在多种组织中创建全面的全长转录组概况.
主要方法:
- 用PacBio长读数和Arima Hi-C链接读数进行基因组组.
- 转录基因组测序利用PacBio对六个关键组织的Iso-Sequencing策略:肌肉,,肝脏,脏,胃和生殖腺.
主要成果:
- 一个1.03 Gb的支架级基因组组件被生成,包括400个支架,N50为33.8 Mb,通过BUSCO基准测试证明97.2%的完整性.
- 全长度转录组分析发现了来自18108个独特基因的56515个异型,N50长度为3973bp.
结论:
- 新生成的基因组和转录组资源为Lutjanus argentimaculatus提供了一个基础数据集.
- 预计这些资源将对该物种的功能研究,保护计划,育种管理和选择性育种计划产生重大益处.
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