相关实验视频

Updated: Jun 20, 2025

Tractable Mammalian Cell Infections with Protozoan-primed Bacteria

Tractable Mammalian Cell Infections with Protozoan-primed Bacteria

Published on: April 2, 2013

拉皮D/拉皮G释放的Legionella pneumophila细胞表面RtxA及其在毒性中的作用

Hussein Kanaan^1,2, Annelise Chapalain¹, Ali Chokr²

¹Centre International de Recherche en Infectiologie (CIRI), Université Lyon 1, INSERM U1111, CNRS UMR5308, ENS, Lyon Bât. Rosalind Franklin, 50 avenue Tony Garnier, Lyon, 69007, France.

BMC microbiology

|July 18, 2024

概括

流感性肺炎菌 (Legionella pneumophila) 是一种流感性肺炎菌.

科学领域:

微生物学微生物学
细菌病原体的产生
蛋白质分泌系统蛋白质分泌系统

背景情况:

肺炎的Legionella导致军团士兵病,一种严重的肺炎.
它产生一个大型RTX蛋白,RtxA,通过1型分泌系统 (T1SS) 分泌.
RTX蛋白与细菌毒性和生物膜形成有关.

研究的目的:

为了研究L. pneumophila RtxA (细胞表面与释放) 的双重定位.
确定RtxA局部化在L. pneumophila病毒性中的作用.

主要方法:

使用LapG蛋白酶进行体外裂解试验.
在缺乏LapG或LapD的L. pneumophila菌株中RtxA局部化的分析.
评估RtxA在阿米巴感染中的作用.

主要成果:

LapG蛋白酶在一个特定的二-氨酸基基因上切割L. pneumophila RtxA.
缺少LapG会在细胞表面保留RtxA;缺少LapD会导致持续释放.
不管其局部位置如何,RtxA都会增强阿米巴细胞感染.

结论:

确定了第一个RTX蛋白分裂部位.
在生物膜形成 (聚合/分散) 中提出的RtxA功能.
RtxA增强了感染,可能是通过宿主细胞相互作用,独立于其局部.

关键词:

在L. Pneumophila的研究中.LapDD LapDD LapDD LapD LapD LapD LapD LapD LapD LapD LapD LapD LapD 在 LapGG 中使用 LapG.RTX家族中的RTX家族.一类分泌系统1型分泌系统病毒性病毒性

更多相关视频

Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages

Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages

Published on: February 22, 2017

Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System

Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System

Published on: March 10, 2020

相关概念视频

Receptor-mediated Endocytosis

Receptor-mediated Endocytosis

Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...

Defense Against Bacterial Pathogens

Defense Against Bacterial Pathogens

The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...