来自牛奶的凝血酶负型葡萄球菌:抗生素图谱和毒性基因检测
Yared Abate Getahun1, Solomon Lulie Abey2, Achenef Melaku Beyene3
1Livestock and Fishery Research Center, Arba Minch University, P.O.BOX: 21, Arba Minch, Ethiopia. abateyared06@gmail.com.
BMC microbiology
|July 18, 2024
概括
埃塞俄比亚牛奶中的凝聚酶阴性葡萄球菌种经常具有多药性耐药性,并携带毒性基因. 这凸显了对这些引起乳内感染的细菌采取控制措施的迫切需要.
科学领域:
- 兽医微生物学 兽医微生物学
- 食品安全 食品安全
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 凝聚酶阴性葡萄球菌 (CoNS) 种类是导致牛乳内感染的原因之一.
- 这些感染带来了重大的经济和公共卫生挑战.
- 关于埃塞俄比亚乳制品农场的CoNS流行,抗生素耐药性和毒性因素的数据有限.
研究的目的:
- 为了确定埃塞俄比亚西北部牛奶中Cons的发生情况.
- 分析孤立的CoNS.的抗生素敏感性模式.
- 为了确定这些CoNS分离物中关键毒性基因的流行率.
主要方法:
- 一项涉及290个牛奶样本的横截面研究.
- 使用标准的微生物学和生物化学技术,对CoNS进行隔离和鉴定.
- 通过Kirby-Bauer盘扩散进行抗菌敏感性测试.
- 使用单复合PCR检测毒性基因.
主要成果:
- 从28.6%的牛奶样本中分离出了CoNS,其中*S. epidermidis*是最常见的物种.
- 所有分离物体对阿莫西西林,安皮西林和塞福泰坦呈现出100%的耐药性.
- 超过一半 (54.2%) 的分离物是多药耐药的,并检测到几个毒性基因 (*icaD*, *pvl*, *mecA*, *hlb*, *sec*, *hla*).
结论:
- 在埃塞俄比亚牛奶中发现了携带毒性和肠毒素基因的多药耐药性CoNS的高患病率.
- 这些发现强调了需要立即实施疾病控制和预防战略的必要性.
- 这是埃塞俄比亚第一个报告CoNS,它们的毒性基因和牛奶中抗生素耐药性的研究.
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