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通过蛋白质阵列分析发现与深静脉血栓形成相关的关键细胞因子

Qitao Wang¹, Junyu Chi¹, Wenjie Zeng¹

¹Vascular Gland Surgery, The First Affiliated Hospital of Hebei North University, Hebei province, Zhangjiakou, 075000, China.

BMC cardiovascular disorders

|July 18, 2024

概括

这项研究确定了12种新的血清生物标志物,包括FGF-6和Galectin-3,用于改善深静脉血栓症 (DVT) 检测. 这些发现为早期诊断和更好地管理DVT提供了潜力.

科学领域:

生物化学和分子生物学
医学诊断医学诊断医学诊断
生物标志物发现发现

背景情况:

静脉血栓栓塞 (VTE) 预测和病因学研究需要扩大生物标志物识别.
早期深静脉血栓 (DVT) 检测和减少并发症需要新的生物标志物和向疗法.

研究的目的:

确定有希望的基于血清的生物标志物,以提高早期DVT的检测准确度.
探索潜在的针对性治疗DVT,并减少相关的并发症.

主要方法:

使用的量子体人类细胞因子抗体阵列440 (QAH-CAA-440) 用于在DVT/非下肢DVT (NDVT) 中进行新型血清生物标志物查.
应用生物信息学用于差异蛋白质分析,随后使用定制阵列和接收器操作特征 (ROC) 分析进行验证.
采用机器学习来开发一个生物标志物模型来评估已识别的目标,选择十二个用于验证.

主要成果:

细胞因子分析确定了12个具有高诊断准确性的关键生物标志物 (AUC从0.773到0.956不等).
关键的验证生物标志物包括FGF-6 (AUC=0.956),加勒-3 (AUC=0.942),EDA-A2 (AUC=0.933),以及CHI3L1 (AUC=0.911).这些生物标志物中,FGF-6是最重要的.
这些细胞因子在区分DVT与NDVT方面表现出显著的潜力.

结论:

EDA-A2,FGF-6,Dkk-4,IL-1 F9,Galectin-3,Layilin,Big-h3,CHI3L1,ULBP-2,Gas-1,IGFBP-5和FGF-9被认为是有前途的生物标志物.这些生物标志物中,FGF-5和FGF-9是最重要的生物标志物.
这些生物标志物代表了DVT诊断和治疗干预的潜在目标.
这项研究强调了细胞因子分析在发现DVT的新生物标志物的有用性.

关键词:

生物标志物生物标志物深静脉血栓是一种深静脉血栓.微阵列蛋白质的结构有针对性的治疗.

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