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生物转化纳米颗粒的绿色合成,表征和功能验证

S Vasanthakumar¹, M Manikandan¹, Muthu Arumugam^1,2

¹Microbial Processes and Technology Division, National Institute for Interdisciplinary Science and Technology (NIIST), Council of Scientific and Industrial Research (CSIR), Trivandrum, Kerala, India.

Biochemistry and biophysics reports

|July 19, 2024

概括

海洋微藻生物合成稳定的元素纳米粒子 (Se-NPs) 用于补充剂. 这种环保方法产生了具有强烈抗氧化活性的Se-NP,非常适合饮食和料应用.

科学领域:

生物技术是生物技术.
纳米技术纳米技术
海洋生物学海洋生物学

背景情况:

是一种必需的微量营养素,具有抗氧化和抗癌性质.
无机是有毒的;有机和元素纳米形式提供更好的生物可用性和较低的毒性.
海洋微藻是生产纳米颗粒 (Se-NPs) 的可持续来源.

研究的目的:

使用海洋微藻优化元素纳米颗粒 (Se-NPs) 的生物合成和生产.
以稳定性和物理性质来表征合成的Se-NP.
验证Se-NPs的生物功能,特别是抗氧化活性.

主要方法:

使用Nanochloropsis oceanica CASA CC201藻类提取物进行Se-NP的生物合成.
优化前体度和藻类提取物比率 (10mM前体,1%提取物,10:1比).
使用原子力显微镜 (AFM),传输电子显微镜 (TEM),拉曼光谱,X射线光电子光谱 (XPS) 和里埃变换红外光谱 (FT-IR) 的表征.

主要成果:

最佳的合成条件产生了高度稳定的Se-NP (183纳米大小,-38.5mV泽塔电位).
AFM和TEM证实了球形,光滑表面,多分散分布的纳米粒子.
FT-IR分析表明微藻蛋白和作为稳定剂和制造剂.
合成的元素纳米形式 (Se0) 显示出显著的激素清除活性 (74%).

结论:

藻类介导的化减少是一种环保,无毒和可持续的Se-NP生产方法.
合成的Se-NP具有高度稳定性,并具有经过验证的生物功能.
这种方法适合大规模生产用于食和料补充剂的Se-NP.

关键词:

抗氧化剂的系统抗氧化剂的系统.生物转化纳米粒子生物转化纳米粒子人类和料补充剂微藻类是一种微藻类.是一种.

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