七通过与裂变酵母细胞运动中的外细胞复合体的物理相互作用在外细胞形成中起作用
Davinder Singh1, Yajun Liu1, Yi-Hua Zhu1
1Department of Molecular Genetics, The Ohio State University, Columbus, Ohio, United States.
bioRxiv : the preprint server for biology
|July 19, 2024
概括
七与外囊复合体物理相互作用,以确保在细胞分裂部位的适当定位. 这种相互作用对于在裂变酵母中的细胞运动过程中指导分泌囊泡至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 细胞骨的动力学
背景情况:
- 七素是细胞骨蛋白质,具有不同的作用,包括支架和膜组织.
- 七对于细胞动力学,细胞分裂过程至关重要,但它们的确切功能尚不清楚.
- 细胞外囊复合体对于在细胞分裂过程中将细胞外囊囊泡与血膜结合至关重要.
研究的目的:
- 为了研究裂变酵母中隔膜和外囊复合体之间的物理相互作用.
- 阐明septins在细胞动力学过程中的外囊复合物的局部化中的作用.
- 了解septin-exocyst相互作用如何影响囊泡向和细胞分离.
主要方法:
- 使用AlphaFold2 ColabFold.使用蛋白质蛋白相互作用的计算预测.
- 使用共免疫沉和酵母两杂交试验进行实验验证.
- 在裂变酵母突变体中分析隔膜和外囊局部化.
主要成果:
- AlphaFold2预测了七和外囊子单元之间的六对直接相互作用.
- 同免疫沉和酵母双杂交试验证实了大多数这些预测的相互作用.
- 七星突变体表现出错位化的外囊复合体,导致由于错误准分泌囊泡,导致细胞分离延迟.
结论:
- 七与外囊复合体直接相互作用,引导其在细胞动力学过程中正确地定位到血边缘.
- 这种隔膜介导的向对于有效的囊泡结合和成功的细胞分离至关重要.
- 这些发现揭示了septins在细胞分裂期间协调细胞骨组织与膜贩运中的关键作用.
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