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Updated: May 2, 2026

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OpenTn5:一个开源资源,用于强大的和可扩展的Tn5转移酶净化和表征

Jan Soroczynski1, Lauren J Anderson1, Joanna L Yeung1

  • 1Laboratory of Genome Architecture and Dynamics, The Rockefeller University, New York, NY.

bioRxiv : the preprint server for biology
|July 19, 2024
PubMed
概括

这项研究介绍了OpenTn5,这是一个开源协议,用于产生超活跃的Tn5转移酶. 这种酶能够有效地对DNA进行标记,用于编排序库的准备,并长期保持活性.

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Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
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