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相关概念视频

Next-generation Sequencing03:00

Next-generation Sequencing

88.6K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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相关实验视频

Updated: Jun 20, 2025

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
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在大规模并行测序平台上进行化Aptamer优化.

Yu-An Kuo, Yuan-I Chen, Yanxing Wang

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    此摘要是机器生成的。

    研究人员使用下一代测序芯片优化了基于DNA的化性受体 (FAP). 一种新型变种,菜/TO1-生物素,证明了显著增强的光特性,用于改进细胞传感应用.

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    科学领域:

    • 生物技术是生物技术.
    • 分子生物学分子生物学
    • 生物物理学的生物物理.

    背景情况:

    • 原性亚体 (FAPs) 对于细胞传感和病原体诊断至关重要.
    • 优化FAP性能是具有挑战性的,即使使用X射线晶体学结构数据.

    研究的目的:

    • 利用下一代测序 (NGS) 芯片开发一种用于优化基于DNA的FAP (D-FAP) 的新方法.
    • 通过选变体并优化其与新染色体的相互作用来提高D-FAP,的性能.

    主要方法:

    • 重新设计的Illumina NGS芯片用于使用TO1-生物素染色体对8,821种菜进行高通量选.
    • 利用分子动态模拟来理解体-染色体相互作用.
    • 通过测量光辐射,量子产量,光寿命和明显解离常数来表征FAP变异.

    主要成果:

    • 用TO1-生物素替换相似的染色体导致53nm红移发射和4倍光增强.
    • 菜中的C14T突变显示了更好的结合亲和力 (较低的Kd),增加了量子产量 (0.62),以及更长的光寿命 (6.00 ns).
    • 优化的C14T变异与原始的菜/TO1-生物素复合体相比,呈现出45%更大的合体光.

    结论:

    • 选和模拟管道有效地优化了FAP,而不需要先前的结构知识.
    • 优化的菜D-FAP与TO1-生物素作为一个改进的分子探测器用于光传感.
    • 这项研究提供了关于 π-π 堆叠在体-染色体复杂结构中的关键作用的见解.