相关实验视频

Updated: Jun 20, 2025

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library
07:28

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

Published on: January 10, 2025

259

高吞吐量变体库和机器学习产量设计规则 复子基因编辑器的设计规则

Kate D Crawford1,2, Asim G Khan1, Santiago C Lopez1,2

  • 1Gladstone Institute of Data Science and Biotechnology, San Francisco, CA, USA.

bioRxiv : the preprint server for biology
|July 19, 2024
PubMed
概括

研究人员优化了细菌逆转录酶系统用于DNA生产. 他们确定了关键的逆子RNA区域进行修改,增强定制DNA合成和改进称为editrons的基因组编辑工具.

更多相关视频

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

1.4K
Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors09:02

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

16.5K

相关概念视频

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.0K